Experimental Design Schematic

Fig.1 Experimental design showing treatments and husbandry events
during the course of the study. Symbols indicate when an experimental
event occurred at each time point. (1) 260 fish were assigned and
acclimated to one of three water temperature groups (e.g., 28°C, 32°C,
or 35°C) and reared from 0 to 164 days post fertilization (dpf). (2a) At
164 dpf (or 0 days post exposure; DPE), fecal collections were collected
from a random selection of five fish per tank (n = 60). Additionally,
histological and wet mount assessments were conducted on selected fish
to assess presence of infection and infection burden. (1b) Afterwards, a
cohort of fish from each water temperature group were exposed to
Pseudocapillaria tomentosa. (3-6) Subsequent fecal samples were
collected and histopathological assessments were conducted at 14 dpe (n
= 54), (3) 21 dpe (n = 48), (4) 28 dpe (n = 47), and (5) 42 dpe (n =
51).
Results
2) Water temperature shapes gut microbiome
structure
Fig. 2 Effects of water temperature on gut microbiomes of parasite
unexposed zebrafish. (A) Simpson’s Index of diversity shows that gut
microbiome diversity significantly differs between fish reared at 28°C
and 35°C water temperatures. (B) Capscale ordination based on the
Bray-Curtis dissimilarity of gut microbiome composition. The analysis
shows that gut microbiome composition significantly differs between fish
reared at different water temperatures. (C) Simpson’s Index of diversity
shows microbial gut diversity increases with time from 0 days post
exposure (dpe) to 42 dpe, irrespective of water temperature. (D)
Capscale ordination of gut microbiome composition based on the
Bray-Curtis dissimilarity constrained on the main effects of water
temperature and time, and their interaction. The analysis shows that
shows that gut microbiome composition differs between fish across time
depending on water temperature. Ribbons and ellipses indicate 95%
confidence interval. Only statistically significant relationships are
shown. A “*” indicates statistical significance below the “0.05” level.
Black arrows indicate direction of greatest change in the indicated
covariates.
2A)

Tables
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GLM
| GLM Results |
| glm(Alpha.Score ~ Temperature); Unexposed fish |
| term |
estimate |
std.error |
statistic |
p.value |
p.adj.sig |
| Shannon |
| (Intercept) |
−0.754 |
0.091 |
−8.309 |
<0.001 |
**** |
| Temperature32 |
0.051 |
0.127 |
0.400 |
≥0.25 |
ns |
| Temperature35 |
0.054 |
0.129 |
0.421 |
≥0.25 |
ns |
| Simpson |
| (Intercept) |
−0.101 |
0.092 |
−1.103 |
≥0.25 |
ns |
| Temperature32 |
0.132 |
0.128 |
1.030 |
≥0.25 |
ns |
| Temperature35 |
0.340 |
0.131 |
2.591 |
0.010 |
* |
| Richness |
| (Intercept) |
−0.231 |
0.075 |
−3.079 |
0.002 |
** |
| Temperature32 |
0.047 |
0.105 |
0.446 |
≥0.25 |
ns |
| Temperature35 |
−0.473 |
0.110 |
−4.299 |
<0.001 |
**** |
ANOVA
| ANOVA of GLM |
| ANOVA(GLM(Alpha.Score ~ Temperature), type = 2); Unexposed fish |
| term |
statistic |
df |
p.value |
sig |
| Shannon |
| Temperature |
0.225 |
2.000 |
≥0.25 |
ns |
| Simpson |
| Temperature |
6.830 |
2.000 |
0.033 |
* |
| Richness |
| Temperature |
27.512 |
2.000 |
<0.001 |
**** |
Tukey
| Pairwise Tukey's HSD, p.adj: Dunnett |
| Tukey(Alpha.Score ~ Temperature); Unexposed fish |
| term |
.y. |
group1 |
group2 |
estimate |
std.error |
statistic |
adj.p.value |
Variable |
| Shannon |
| Temperature |
Alpha.Score |
32 |
28 |
0.051 |
0.127 |
0.400 |
≥0.25 |
Temperature |
| Temperature |
Alpha.Score |
35 |
28 |
0.054 |
0.129 |
0.421 |
≥0.25 |
Temperature |
| Temperature |
Alpha.Score |
35 |
32 |
0.004 |
0.127 |
0.029 |
≥0.25 |
Temperature |
| Simpson |
| Temperature |
Alpha.Score |
32 |
28 |
0.132 |
0.128 |
1.030 |
≥0.25 |
Temperature |
| Temperature |
Alpha.Score |
35 |
28 |
0.340 |
0.131 |
2.591 |
0.026 |
Temperature |
| Temperature |
Alpha.Score |
35 |
32 |
0.207 |
0.130 |
1.596 |
0.247 |
Temperature |
| Richness |
| Temperature |
Alpha.Score |
32 |
28 |
0.047 |
0.105 |
0.446 |
≥0.25 |
Temperature |
| Temperature |
Alpha.Score |
35 |
28 |
−0.473 |
0.110 |
−4.299 |
<0.001 |
Temperature |
| Temperature |
Alpha.Score |
35 |
32 |
−0.520 |
0.109 |
−4.768 |
<0.001 |
Temperature |
2B)

Tables
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Capscale
| ADONIS2 |
| adonis2(Beta Distance ~ Temperature); Unexposed fish |
| term |
df |
SumOfSqs |
R2 |
statistic |
p.value |
sig |
| bray |
| Temperature |
2.000 |
2.294 |
0.133 |
12.900 |
0.001 |
*** |
| Residual |
168.000 |
14.936 |
0.867 |
NA |
NA |
NA |
| Total |
170.000 |
17.229 |
1.000 |
NA |
NA |
NA |
| canberra |
| Temperature |
2.000 |
3.862 |
0.099 |
9.178 |
0.001 |
*** |
| Residual |
168.000 |
35.344 |
0.901 |
NA |
NA |
NA |
| Total |
170.000 |
39.206 |
1.000 |
NA |
NA |
NA |
| gunifrac |
| Temperature |
2.000 |
1.422 |
0.102 |
9.566 |
0.001 |
*** |
| Residual |
168.000 |
12.485 |
0.898 |
NA |
NA |
NA |
| Total |
170.000 |
13.907 |
1.000 |
NA |
NA |
NA |
Dispersion (ANOVA)
| ANOVA: Homogeneity of Dispersion |
| ANOVA(Beta Disperson ~ Temperature); Unexposed fish |
| term |
df |
sumsq |
meansq |
statistic |
p.value |
sig |
| bray |
| Temperature |
2.000 |
0.008 |
0.004 |
0.386 |
≥0.25 |
ns |
| Residual |
168.000 |
1.771 |
0.011 |
NA |
NA |
NA |
| canberra |
| Temperature |
2.000 |
0.008 |
0.004 |
0.873 |
≥0.25 |
ns |
| Residual |
168.000 |
0.759 |
0.005 |
NA |
NA |
NA |
| gunifrac |
| Temperature |
2.000 |
0.005 |
0.003 |
0.369 |
≥0.25 |
ns |
| Residual |
168.000 |
1.143 |
0.007 |
NA |
NA |
NA |
Dispersion (Tukey)
| Tukey: Homogeneity of Dispersion |
| Tukey(Beta Disperson ~ Temperature); Unexposed fish |
| .y. |
term |
group1 |
group2 |
estimate |
conf.low |
conf.high |
adj.p.value |
sig |
| bray |
| Distance |
Temperature |
32 |
28 |
−0.011 |
−0.056 |
0.034 |
≥0.25 |
ns |
| Distance |
Temperature |
35 |
28 |
0.005 |
−0.041 |
0.051 |
≥0.25 |
ns |
| Distance |
Temperature |
35 |
32 |
0.016 |
−0.029 |
0.062 |
≥0.25 |
ns |
| canberra |
| Distance |
Temperature |
32 |
28 |
−0.001 |
−0.031 |
0.028 |
≥0.25 |
ns |
| Distance |
Temperature |
35 |
28 |
0.014 |
−0.016 |
0.044 |
≥0.25 |
ns |
| Distance |
Temperature |
35 |
32 |
0.015 |
−0.015 |
0.045 |
≥0.25 |
ns |
| gunifrac |
| Distance |
Temperature |
32 |
28 |
−0.013 |
−0.049 |
0.023 |
≥0.25 |
ns |
| Distance |
Temperature |
35 |
28 |
−0.004 |
−0.040 |
0.033 |
≥0.25 |
ns |
| Distance |
Temperature |
35 |
32 |
0.009 |
−0.027 |
0.046 |
≥0.25 |
ns |
2C)

Tables
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GLM
| GLM Results |
| glm(Alpha.Score ~ Temperature*DPE); Unexposed fish |
| term |
estimate |
std.error |
statistic |
p.value |
p.adj.sig |
| Shannon |
| (Intercept) |
−1.074 |
0.138 |
−7.754 |
<0.001 |
**** |
| Temperature32 |
0.075 |
0.193 |
0.386 |
≥0.25 |
ns |
| Temperature35 |
0.154 |
0.193 |
0.797 |
≥0.25 |
ns |
| DPE |
0.018 |
0.006 |
3.086 |
0.002 |
** |
| Temperature32:DPE |
−0.001 |
0.008 |
−0.170 |
≥0.25 |
ns |
| Temperature35:DPE |
−0.005 |
0.008 |
−0.585 |
≥0.25 |
ns |
| Simpson |
| (Intercept) |
−0.433 |
0.134 |
−3.245 |
0.001 |
** |
| Temperature32 |
0.157 |
0.186 |
0.841 |
≥0.25 |
ns |
| Temperature35 |
0.408 |
0.187 |
2.177 |
0.031 |
* |
| DPE |
0.019 |
0.006 |
3.293 |
0.001 |
** |
| Temperature32:DPE |
−0.001 |
0.008 |
−0.173 |
≥0.25 |
ns |
| Temperature35:DPE |
−0.003 |
0.008 |
−0.343 |
≥0.25 |
ns |
| Richness |
| (Intercept) |
−0.651 |
0.108 |
−6.017 |
<0.001 |
**** |
| Temperature32 |
0.189 |
0.150 |
1.257 |
0.211 |
ns |
| Temperature35 |
−0.059 |
0.154 |
−0.383 |
≥0.25 |
ns |
| DPE |
0.024 |
0.005 |
5.137 |
<0.001 |
**** |
| Temperature32:DPE |
−0.008 |
0.006 |
−1.251 |
0.213 |
ns |
| Temperature35:DPE |
−0.024 |
0.007 |
−3.476 |
<0.001 |
*** |
ANOVA
| ANOVA of GLM |
| ANOVA(GLM(Alpha.Score ~ Temperature*Time), type = 2); Unexposed fish |
| term |
statistic |
df |
p.value |
sig |
| Shannon |
| Temperature |
0.322 |
2.000 |
≥0.25 |
ns |
| DPE |
22.895 |
1.000 |
<0.001 |
**** |
| Temperature:DPE |
0.363 |
2.000 |
≥0.25 |
ns |
| Simpson |
| Temperature |
8.551 |
2.000 |
0.014 |
* |
| DPE |
28.161 |
1.000 |
<0.001 |
**** |
| Temperature:DPE |
0.118 |
2.000 |
≥0.25 |
ns |
| Richness |
| Temperature |
31.759 |
2.000 |
<0.001 |
**** |
| DPE |
27.201 |
1.000 |
<0.001 |
**** |
| Temperature:DPE |
12.460 |
2.000 |
0.002 |
** |
Tukey
| Pairwise Tukey's HSD, p.adj: Dunnett |
| Tukey(Alpha.Score ~ Temperature*Time); Unexposed fish |
| Temperature |
.y. |
term |
group1 |
group2 |
estimate |
std.error |
statistic |
adj.p.value |
Variable |
Group |
| Shannon |
| 28 |
Alpha.Score |
DPE |
14 |
0 |
1.065 |
0.294 |
3.625 |
0.003 |
DPE |
28 |
| 28 |
Alpha.Score |
DPE |
21 |
0 |
0.722 |
0.281 |
2.566 |
0.076 |
DPE |
28 |
| 28 |
Alpha.Score |
DPE |
28 |
0 |
1.187 |
0.274 |
4.335 |
<0.001 |
DPE |
28 |
| 28 |
Alpha.Score |
DPE |
42 |
0 |
0.548 |
0.296 |
1.853 |
≥0.25 |
DPE |
28 |
| 28 |
Alpha.Score |
DPE |
21 |
14 |
−0.343 |
0.317 |
−1.083 |
≥0.25 |
DPE |
28 |
| 28 |
Alpha.Score |
DPE |
28 |
14 |
0.122 |
0.310 |
0.392 |
≥0.25 |
DPE |
28 |
| 28 |
Alpha.Score |
DPE |
42 |
14 |
−0.517 |
0.330 |
−1.567 |
≥0.25 |
DPE |
28 |
| 28 |
Alpha.Score |
DPE |
28 |
21 |
0.465 |
0.299 |
1.558 |
≥0.25 |
DPE |
28 |
| 28 |
Alpha.Score |
DPE |
42 |
21 |
−0.174 |
0.319 |
−0.545 |
≥0.25 |
DPE |
28 |
| 28 |
Alpha.Score |
DPE |
42 |
28 |
−0.639 |
0.312 |
−2.045 |
0.243 |
DPE |
28 |
| 32 |
Alpha.Score |
DPE |
14 |
0 |
0.006 |
0.250 |
0.022 |
≥0.25 |
DPE |
32 |
| 32 |
Alpha.Score |
DPE |
21 |
0 |
0.112 |
0.247 |
0.452 |
≥0.25 |
DPE |
32 |
| 32 |
Alpha.Score |
DPE |
28 |
0 |
0.420 |
0.248 |
1.695 |
≥0.25 |
DPE |
32 |
| 32 |
Alpha.Score |
DPE |
42 |
0 |
0.694 |
0.236 |
2.943 |
0.027 |
DPE |
32 |
| 32 |
Alpha.Score |
DPE |
21 |
14 |
0.106 |
0.286 |
0.371 |
≥0.25 |
DPE |
32 |
| 32 |
Alpha.Score |
DPE |
28 |
14 |
0.414 |
0.287 |
1.445 |
≥0.25 |
DPE |
32 |
| 32 |
Alpha.Score |
DPE |
42 |
14 |
0.688 |
0.276 |
2.490 |
0.092 |
DPE |
32 |
| 32 |
Alpha.Score |
DPE |
28 |
21 |
0.308 |
0.283 |
1.087 |
≥0.25 |
DPE |
32 |
| 32 |
Alpha.Score |
DPE |
42 |
21 |
0.582 |
0.273 |
2.131 |
0.205 |
DPE |
32 |
| 32 |
Alpha.Score |
DPE |
42 |
28 |
0.274 |
0.274 |
1.001 |
≥0.25 |
DPE |
32 |
| 35 |
Alpha.Score |
DPE |
14 |
0 |
−0.006 |
0.210 |
−0.027 |
≥0.25 |
DPE |
35 |
| 35 |
Alpha.Score |
DPE |
21 |
0 |
−0.249 |
0.218 |
−1.145 |
≥0.25 |
DPE |
35 |
| 35 |
Alpha.Score |
DPE |
28 |
0 |
0.232 |
0.204 |
1.138 |
≥0.25 |
DPE |
35 |
| 35 |
Alpha.Score |
DPE |
42 |
0 |
0.661 |
0.207 |
3.194 |
0.012 |
DPE |
35 |
| 35 |
Alpha.Score |
DPE |
21 |
14 |
−0.243 |
0.253 |
−0.962 |
≥0.25 |
DPE |
35 |
| 35 |
Alpha.Score |
DPE |
28 |
14 |
0.238 |
0.242 |
0.985 |
≥0.25 |
DPE |
35 |
| 35 |
Alpha.Score |
DPE |
42 |
14 |
0.667 |
0.244 |
2.733 |
0.049 |
DPE |
35 |
| 35 |
Alpha.Score |
DPE |
28 |
21 |
0.481 |
0.248 |
1.938 |
≥0.25 |
DPE |
35 |
| 35 |
Alpha.Score |
DPE |
42 |
21 |
0.910 |
0.251 |
3.628 |
0.003 |
DPE |
35 |
| 35 |
Alpha.Score |
DPE |
42 |
28 |
0.429 |
0.239 |
1.792 |
≥0.25 |
DPE |
35 |
| Simpson |
| 28 |
Alpha.Score |
DPE |
14 |
0 |
1.256 |
0.273 |
4.611 |
<0.001 |
DPE |
28 |
| 28 |
Alpha.Score |
DPE |
21 |
0 |
0.761 |
0.247 |
3.076 |
0.018 |
DPE |
28 |
| 28 |
Alpha.Score |
DPE |
28 |
0 |
1.334 |
0.254 |
5.241 |
<0.001 |
DPE |
28 |
| 28 |
Alpha.Score |
DPE |
42 |
0 |
0.514 |
0.257 |
2.002 |
≥0.25 |
DPE |
28 |
| 28 |
Alpha.Score |
DPE |
21 |
14 |
−0.496 |
0.302 |
−1.644 |
≥0.25 |
DPE |
28 |
| 28 |
Alpha.Score |
DPE |
28 |
14 |
0.077 |
0.308 |
0.251 |
≥0.25 |
DPE |
28 |
| 28 |
Alpha.Score |
DPE |
42 |
14 |
−0.742 |
0.310 |
−2.398 |
0.114 |
DPE |
28 |
| 28 |
Alpha.Score |
DPE |
28 |
21 |
0.573 |
0.285 |
2.008 |
≥0.25 |
DPE |
28 |
| 28 |
Alpha.Score |
DPE |
42 |
21 |
−0.246 |
0.288 |
−0.857 |
≥0.25 |
DPE |
28 |
| 28 |
Alpha.Score |
DPE |
42 |
28 |
−0.819 |
0.294 |
−2.789 |
0.042 |
DPE |
28 |
| 32 |
Alpha.Score |
DPE |
14 |
0 |
0.065 |
0.249 |
0.261 |
≥0.25 |
DPE |
32 |
| 32 |
Alpha.Score |
DPE |
21 |
0 |
0.142 |
0.249 |
0.569 |
≥0.25 |
DPE |
32 |
| 32 |
Alpha.Score |
DPE |
28 |
0 |
0.592 |
0.261 |
2.272 |
0.152 |
DPE |
32 |
| 32 |
Alpha.Score |
DPE |
42 |
0 |
0.724 |
0.254 |
2.850 |
0.035 |
DPE |
32 |
| 32 |
Alpha.Score |
DPE |
21 |
14 |
0.077 |
0.287 |
0.267 |
≥0.25 |
DPE |
32 |
| 32 |
Alpha.Score |
DPE |
28 |
14 |
0.527 |
0.297 |
1.773 |
≥0.25 |
DPE |
32 |
| 32 |
Alpha.Score |
DPE |
42 |
14 |
0.659 |
0.292 |
2.260 |
0.156 |
DPE |
32 |
| 32 |
Alpha.Score |
DPE |
28 |
21 |
0.451 |
0.297 |
1.517 |
≥0.25 |
DPE |
32 |
| 32 |
Alpha.Score |
DPE |
42 |
21 |
0.582 |
0.291 |
1.999 |
≥0.25 |
DPE |
32 |
| 32 |
Alpha.Score |
DPE |
42 |
28 |
0.132 |
0.302 |
0.436 |
≥0.25 |
DPE |
32 |
| 35 |
Alpha.Score |
DPE |
14 |
0 |
0.149 |
0.219 |
0.683 |
≥0.25 |
DPE |
35 |
| 35 |
Alpha.Score |
DPE |
21 |
0 |
−0.167 |
0.218 |
−0.766 |
≥0.25 |
DPE |
35 |
| 35 |
Alpha.Score |
DPE |
28 |
0 |
0.413 |
0.222 |
1.858 |
≥0.25 |
DPE |
35 |
| 35 |
Alpha.Score |
DPE |
42 |
0 |
0.836 |
0.244 |
3.431 |
0.005 |
DPE |
35 |
| 35 |
Alpha.Score |
DPE |
21 |
14 |
−0.316 |
0.257 |
−1.232 |
≥0.25 |
DPE |
35 |
| 35 |
Alpha.Score |
DPE |
28 |
14 |
0.263 |
0.260 |
1.013 |
≥0.25 |
DPE |
35 |
| 35 |
Alpha.Score |
DPE |
42 |
14 |
0.687 |
0.279 |
2.465 |
0.097 |
DPE |
35 |
| 35 |
Alpha.Score |
DPE |
28 |
21 |
0.579 |
0.260 |
2.233 |
0.165 |
DPE |
35 |
| 35 |
Alpha.Score |
DPE |
42 |
21 |
1.003 |
0.278 |
3.604 |
0.003 |
DPE |
35 |
| 35 |
Alpha.Score |
DPE |
42 |
28 |
0.424 |
0.282 |
1.506 |
≥0.25 |
DPE |
35 |
| Richness |
| 28 |
Alpha.Score |
DPE |
14 |
0 |
0.432 |
0.236 |
1.836 |
≥0.25 |
DPE |
28 |
| 28 |
Alpha.Score |
DPE |
21 |
0 |
0.550 |
0.218 |
2.526 |
0.084 |
DPE |
28 |
| 28 |
Alpha.Score |
DPE |
28 |
0 |
0.767 |
0.218 |
3.525 |
0.004 |
DPE |
28 |
| 28 |
Alpha.Score |
DPE |
42 |
0 |
0.970 |
0.227 |
4.278 |
<0.001 |
DPE |
28 |
| 28 |
Alpha.Score |
DPE |
21 |
14 |
0.118 |
0.263 |
0.449 |
≥0.25 |
DPE |
28 |
| 28 |
Alpha.Score |
DPE |
28 |
14 |
0.335 |
0.262 |
1.275 |
≥0.25 |
DPE |
28 |
| 28 |
Alpha.Score |
DPE |
42 |
14 |
0.537 |
0.270 |
1.990 |
≥0.25 |
DPE |
28 |
| 28 |
Alpha.Score |
DPE |
28 |
21 |
0.217 |
0.247 |
0.880 |
≥0.25 |
DPE |
28 |
| 28 |
Alpha.Score |
DPE |
42 |
21 |
0.419 |
0.255 |
1.647 |
≥0.25 |
DPE |
28 |
| 28 |
Alpha.Score |
DPE |
42 |
28 |
0.203 |
0.254 |
0.796 |
≥0.25 |
DPE |
28 |
| 32 |
Alpha.Score |
DPE |
14 |
0 |
−0.170 |
0.177 |
−0.965 |
≥0.25 |
DPE |
32 |
| 32 |
Alpha.Score |
DPE |
21 |
0 |
−0.026 |
0.175 |
−0.147 |
≥0.25 |
DPE |
32 |
| 32 |
Alpha.Score |
DPE |
28 |
0 |
0.187 |
0.180 |
1.041 |
≥0.25 |
DPE |
32 |
| 32 |
Alpha.Score |
DPE |
42 |
0 |
0.769 |
0.176 |
4.359 |
<0.001 |
DPE |
32 |
| 32 |
Alpha.Score |
DPE |
21 |
14 |
0.145 |
0.204 |
0.711 |
≥0.25 |
DPE |
32 |
| 32 |
Alpha.Score |
DPE |
28 |
14 |
0.358 |
0.208 |
1.721 |
≥0.25 |
DPE |
32 |
| 32 |
Alpha.Score |
DPE |
42 |
14 |
0.939 |
0.205 |
4.585 |
<0.001 |
DPE |
32 |
| 32 |
Alpha.Score |
DPE |
28 |
21 |
0.213 |
0.206 |
1.031 |
≥0.25 |
DPE |
32 |
| 32 |
Alpha.Score |
DPE |
42 |
21 |
0.794 |
0.203 |
3.906 |
<0.001 |
DPE |
32 |
| 32 |
Alpha.Score |
DPE |
42 |
28 |
0.581 |
0.208 |
2.801 |
0.040 |
DPE |
32 |
| 35 |
Alpha.Score |
DPE |
14 |
0 |
−0.560 |
0.214 |
−2.612 |
0.067 |
DPE |
35 |
| 35 |
Alpha.Score |
DPE |
21 |
0 |
−0.551 |
0.214 |
−2.578 |
0.073 |
DPE |
35 |
| 35 |
Alpha.Score |
DPE |
28 |
0 |
0.110 |
0.196 |
0.561 |
≥0.25 |
DPE |
35 |
| 35 |
Alpha.Score |
DPE |
42 |
0 |
0.007 |
0.206 |
0.036 |
≥0.25 |
DPE |
35 |
| 35 |
Alpha.Score |
DPE |
21 |
14 |
0.008 |
0.259 |
0.032 |
≥0.25 |
DPE |
35 |
| 35 |
Alpha.Score |
DPE |
28 |
14 |
0.670 |
0.245 |
2.733 |
0.048 |
DPE |
35 |
| 35 |
Alpha.Score |
DPE |
42 |
14 |
0.567 |
0.253 |
2.240 |
0.162 |
DPE |
35 |
| 35 |
Alpha.Score |
DPE |
28 |
21 |
0.661 |
0.245 |
2.702 |
0.053 |
DPE |
35 |
| 35 |
Alpha.Score |
DPE |
42 |
21 |
0.559 |
0.253 |
2.210 |
0.173 |
DPE |
35 |
| 35 |
Alpha.Score |
DPE |
42 |
28 |
−0.103 |
0.238 |
−0.431 |
≥0.25 |
DPE |
35 |
2D)

Tables
(hide)
Press on tabs to display tables. Scroll to see additional rows.
Capscale
| ADONIS2 |
| adonis2(Beta Distance ~ Temperature*DPE); Unexposed fish |
| term |
df |
SumOfSqs |
R2 |
statistic |
p.value |
sig |
| bray |
| Temperature |
2.000 |
2.294 |
0.133 |
16.443 |
0.001 |
*** |
| DPE |
1.000 |
2.531 |
0.147 |
36.284 |
0.001 |
*** |
| Temperature:DPE |
2.000 |
0.896 |
0.052 |
6.425 |
0.001 |
*** |
| Residual |
165.000 |
11.509 |
0.668 |
NA |
NA |
NA |
| Total |
170.000 |
17.229 |
1.000 |
NA |
NA |
NA |
| canberra |
| Temperature |
2.000 |
3.862 |
0.099 |
10.667 |
0.001 |
*** |
| DPE |
1.000 |
3.655 |
0.093 |
20.192 |
0.001 |
*** |
| Temperature:DPE |
2.000 |
1.820 |
0.046 |
5.026 |
0.001 |
*** |
| Residual |
165.000 |
29.869 |
0.762 |
NA |
NA |
NA |
| Total |
170.000 |
39.206 |
1.000 |
NA |
NA |
NA |
| gunifrac |
| Temperature |
2.000 |
1.422 |
0.102 |
11.984 |
0.001 |
*** |
| DPE |
1.000 |
2.123 |
0.153 |
35.785 |
0.001 |
*** |
| Temperature:DPE |
2.000 |
0.574 |
0.041 |
4.840 |
0.001 |
*** |
| Residual |
165.000 |
9.788 |
0.704 |
NA |
NA |
NA |
| Total |
170.000 |
13.907 |
1.000 |
NA |
NA |
NA |
Dispersion (ANOVA)
| ANOVA: Homogeneity of Dispersion |
| ANOVA(Beta Disperson ~ Temperature); Unexposed fish |
| term |
df |
sumsq |
meansq |
statistic |
p.value |
sig |
| bray |
| Temp.DPE |
14.000 |
0.290 |
0.021 |
2.348 |
0.006 |
** |
| Residual |
156.000 |
1.376 |
0.009 |
NA |
NA |
NA |
| canberra |
| Temp.DPE |
14.000 |
0.250 |
0.018 |
3.344 |
<0.001 |
*** |
| Residual |
156.000 |
0.831 |
0.005 |
NA |
NA |
NA |
| gunifrac |
| Temp.DPE |
14.000 |
0.249 |
0.018 |
2.986 |
<0.001 |
*** |
| Residual |
156.000 |
0.929 |
0.006 |
NA |
NA |
NA |
Dispersion (Tukey)
| Tukey: Homogeneity of Dispersion |
| Tukey(Beta Disperson ~ Temperature*DPE); Unexposed fish |
| .y. |
term |
group1 |
group2 |
estimate |
conf.low |
conf.high |
adj.p.value |
sig |
| bray |
| Distance |
Temp.DPE |
28°C__14DPE |
28°C__0DPE |
0.024 |
−0.112 |
0.159 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
28°C__21DPE |
28°C__0DPE |
0.052 |
−0.074 |
0.177 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
28°C__28DPE |
28°C__0DPE |
0.007 |
−0.118 |
0.133 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
28°C__42DPE |
28°C__0DPE |
−0.017 |
−0.147 |
0.113 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__0DPE |
28°C__0DPE |
0.097 |
−0.005 |
0.199 |
0.085 |
ns |
| Distance |
Temp.DPE |
32°C__14DPE |
28°C__0DPE |
0.041 |
−0.085 |
0.166 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__21DPE |
28°C__0DPE |
−0.018 |
−0.143 |
0.108 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__28DPE |
28°C__0DPE |
0.028 |
−0.102 |
0.158 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__42DPE |
28°C__0DPE |
0.017 |
−0.108 |
0.142 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__0DPE |
28°C__0DPE |
0.044 |
−0.058 |
0.146 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__14DPE |
28°C__0DPE |
0.004 |
−0.126 |
0.134 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__21DPE |
28°C__0DPE |
−0.004 |
−0.134 |
0.126 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__28DPE |
28°C__0DPE |
0.033 |
−0.097 |
0.162 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__42DPE |
28°C__0DPE |
0.146 |
0.011 |
0.282 |
0.021 |
* |
| Distance |
Temp.DPE |
28°C__21DPE |
28°C__14DPE |
0.028 |
−0.126 |
0.181 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
28°C__28DPE |
28°C__14DPE |
−0.016 |
−0.170 |
0.137 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
28°C__42DPE |
28°C__14DPE |
−0.040 |
−0.198 |
0.117 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__0DPE |
28°C__14DPE |
0.073 |
−0.062 |
0.209 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__14DPE |
28°C__14DPE |
0.017 |
−0.136 |
0.171 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__21DPE |
28°C__14DPE |
−0.041 |
−0.195 |
0.112 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__28DPE |
28°C__14DPE |
0.005 |
−0.153 |
0.162 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__42DPE |
28°C__14DPE |
−0.006 |
−0.160 |
0.147 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__0DPE |
28°C__14DPE |
0.020 |
−0.115 |
0.156 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__14DPE |
28°C__14DPE |
−0.019 |
−0.176 |
0.138 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__21DPE |
28°C__14DPE |
−0.028 |
−0.185 |
0.129 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__28DPE |
28°C__14DPE |
0.009 |
−0.148 |
0.166 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__42DPE |
28°C__14DPE |
0.123 |
−0.039 |
0.284 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
28°C__28DPE |
28°C__21DPE |
−0.044 |
−0.189 |
0.101 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
28°C__42DPE |
28°C__21DPE |
−0.068 |
−0.217 |
0.081 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__0DPE |
28°C__21DPE |
0.045 |
−0.080 |
0.171 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__14DPE |
28°C__21DPE |
−0.011 |
−0.156 |
0.134 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__21DPE |
28°C__21DPE |
−0.069 |
−0.214 |
0.076 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__28DPE |
28°C__21DPE |
−0.023 |
−0.172 |
0.125 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__42DPE |
28°C__21DPE |
−0.034 |
−0.179 |
0.110 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__0DPE |
28°C__21DPE |
−0.008 |
−0.133 |
0.118 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__14DPE |
28°C__21DPE |
−0.047 |
−0.196 |
0.102 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__21DPE |
28°C__21DPE |
−0.056 |
−0.205 |
0.093 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__28DPE |
28°C__21DPE |
−0.019 |
−0.168 |
0.130 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__42DPE |
28°C__21DPE |
0.095 |
−0.059 |
0.248 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
28°C__42DPE |
28°C__28DPE |
−0.024 |
−0.173 |
0.125 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__0DPE |
28°C__28DPE |
0.089 |
−0.036 |
0.215 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__14DPE |
28°C__28DPE |
0.033 |
−0.111 |
0.178 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__21DPE |
28°C__28DPE |
−0.025 |
−0.170 |
0.120 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__28DPE |
28°C__28DPE |
0.021 |
−0.128 |
0.169 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__42DPE |
28°C__28DPE |
0.010 |
−0.135 |
0.154 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__0DPE |
28°C__28DPE |
0.036 |
−0.089 |
0.162 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__14DPE |
28°C__28DPE |
−0.003 |
−0.152 |
0.146 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__21DPE |
28°C__28DPE |
−0.012 |
−0.161 |
0.137 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__28DPE |
28°C__28DPE |
0.025 |
−0.124 |
0.174 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__42DPE |
28°C__28DPE |
0.139 |
−0.015 |
0.292 |
0.125 |
ns |
| Distance |
Temp.DPE |
32°C__0DPE |
28°C__42DPE |
0.114 |
−0.016 |
0.243 |
0.161 |
ns |
| Distance |
Temp.DPE |
32°C__14DPE |
28°C__42DPE |
0.057 |
−0.091 |
0.206 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__21DPE |
28°C__42DPE |
−0.001 |
−0.150 |
0.148 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__28DPE |
28°C__42DPE |
0.045 |
−0.108 |
0.197 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__42DPE |
28°C__42DPE |
0.034 |
−0.115 |
0.183 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__0DPE |
28°C__42DPE |
0.061 |
−0.069 |
0.191 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__14DPE |
28°C__42DPE |
0.021 |
−0.131 |
0.174 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__21DPE |
28°C__42DPE |
0.012 |
−0.140 |
0.165 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__28DPE |
28°C__42DPE |
0.049 |
−0.103 |
0.202 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__42DPE |
28°C__42DPE |
0.163 |
0.006 |
0.320 |
0.034 |
* |
| Distance |
Temp.DPE |
32°C__14DPE |
32°C__0DPE |
−0.056 |
−0.182 |
0.069 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__21DPE |
32°C__0DPE |
−0.114 |
−0.240 |
0.011 |
0.115 |
ns |
| Distance |
Temp.DPE |
32°C__28DPE |
32°C__0DPE |
−0.069 |
−0.199 |
0.061 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__42DPE |
32°C__0DPE |
−0.080 |
−0.205 |
0.046 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__0DPE |
32°C__0DPE |
−0.053 |
−0.155 |
0.049 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__14DPE |
32°C__0DPE |
−0.092 |
−0.222 |
0.037 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__21DPE |
32°C__0DPE |
−0.101 |
−0.231 |
0.029 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__28DPE |
32°C__0DPE |
−0.064 |
−0.194 |
0.066 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__42DPE |
32°C__0DPE |
0.049 |
−0.086 |
0.185 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__21DPE |
32°C__14DPE |
−0.058 |
−0.203 |
0.086 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__28DPE |
32°C__14DPE |
−0.013 |
−0.161 |
0.136 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__42DPE |
32°C__14DPE |
−0.024 |
−0.168 |
0.121 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__0DPE |
32°C__14DPE |
0.003 |
−0.122 |
0.129 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__14DPE |
32°C__14DPE |
−0.036 |
−0.185 |
0.112 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__21DPE |
32°C__14DPE |
−0.045 |
−0.194 |
0.104 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__28DPE |
32°C__14DPE |
−0.008 |
−0.157 |
0.141 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__42DPE |
32°C__14DPE |
0.105 |
−0.048 |
0.259 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__28DPE |
32°C__21DPE |
0.046 |
−0.103 |
0.194 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__42DPE |
32°C__21DPE |
0.035 |
−0.110 |
0.179 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__0DPE |
32°C__21DPE |
0.061 |
−0.064 |
0.187 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__14DPE |
32°C__21DPE |
0.022 |
−0.127 |
0.171 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__21DPE |
32°C__21DPE |
0.013 |
−0.136 |
0.162 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__28DPE |
32°C__21DPE |
0.050 |
−0.099 |
0.199 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__42DPE |
32°C__21DPE |
0.164 |
0.010 |
0.317 |
0.025 |
* |
| Distance |
Temp.DPE |
32°C__42DPE |
32°C__28DPE |
−0.011 |
−0.160 |
0.138 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__0DPE |
32°C__28DPE |
0.016 |
−0.114 |
0.146 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__14DPE |
32°C__28DPE |
−0.024 |
−0.176 |
0.129 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__21DPE |
32°C__28DPE |
−0.033 |
−0.185 |
0.120 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__28DPE |
32°C__28DPE |
0.004 |
−0.148 |
0.157 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__42DPE |
32°C__28DPE |
0.118 |
−0.039 |
0.275 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__0DPE |
32°C__42DPE |
0.027 |
−0.099 |
0.152 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__14DPE |
32°C__42DPE |
−0.013 |
−0.161 |
0.136 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__21DPE |
32°C__42DPE |
−0.021 |
−0.170 |
0.127 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__28DPE |
32°C__42DPE |
0.015 |
−0.133 |
0.164 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__42DPE |
32°C__42DPE |
0.129 |
−0.024 |
0.283 |
0.209 |
ns |
| Distance |
Temp.DPE |
35°C__14DPE |
35°C__0DPE |
−0.039 |
−0.169 |
0.090 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__21DPE |
35°C__0DPE |
−0.048 |
−0.178 |
0.082 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__28DPE |
35°C__0DPE |
−0.011 |
−0.141 |
0.119 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__42DPE |
35°C__0DPE |
0.102 |
−0.033 |
0.238 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__21DPE |
35°C__14DPE |
−0.009 |
−0.161 |
0.144 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__28DPE |
35°C__14DPE |
0.028 |
−0.124 |
0.181 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__42DPE |
35°C__14DPE |
0.142 |
−0.016 |
0.299 |
0.127 |
ns |
| Distance |
Temp.DPE |
35°C__28DPE |
35°C__21DPE |
0.037 |
−0.116 |
0.190 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__42DPE |
35°C__21DPE |
0.151 |
−0.007 |
0.308 |
0.076 |
ns |
| Distance |
Temp.DPE |
35°C__42DPE |
35°C__28DPE |
0.114 |
−0.044 |
0.271 |
≥0.25 |
ns |
| canberra |
| Distance |
Temp.DPE |
28°C__14DPE |
28°C__0DPE |
−0.033 |
−0.138 |
0.072 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
28°C__21DPE |
28°C__0DPE |
−0.005 |
−0.103 |
0.092 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
28°C__28DPE |
28°C__0DPE |
−0.074 |
−0.171 |
0.024 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
28°C__42DPE |
28°C__0DPE |
−0.069 |
−0.170 |
0.032 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__0DPE |
28°C__0DPE |
0.035 |
−0.045 |
0.115 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__14DPE |
28°C__0DPE |
−0.021 |
−0.119 |
0.076 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__21DPE |
28°C__0DPE |
−0.070 |
−0.167 |
0.028 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__28DPE |
28°C__0DPE |
−0.056 |
−0.157 |
0.045 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__42DPE |
28°C__0DPE |
−0.068 |
−0.165 |
0.030 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__0DPE |
28°C__0DPE |
0.008 |
−0.071 |
0.088 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__14DPE |
28°C__0DPE |
−0.041 |
−0.142 |
0.060 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__21DPE |
28°C__0DPE |
−0.047 |
−0.148 |
0.054 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__28DPE |
28°C__0DPE |
−0.052 |
−0.153 |
0.049 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__42DPE |
28°C__0DPE |
0.045 |
−0.061 |
0.150 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
28°C__21DPE |
28°C__14DPE |
0.028 |
−0.091 |
0.147 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
28°C__28DPE |
28°C__14DPE |
−0.040 |
−0.160 |
0.079 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
28°C__42DPE |
28°C__14DPE |
−0.035 |
−0.158 |
0.087 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__0DPE |
28°C__14DPE |
0.068 |
−0.037 |
0.174 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__14DPE |
28°C__14DPE |
0.012 |
−0.107 |
0.131 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__21DPE |
28°C__14DPE |
−0.037 |
−0.156 |
0.083 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__28DPE |
28°C__14DPE |
−0.023 |
−0.145 |
0.099 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__42DPE |
28°C__14DPE |
−0.034 |
−0.154 |
0.085 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__0DPE |
28°C__14DPE |
0.042 |
−0.064 |
0.147 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__14DPE |
28°C__14DPE |
−0.008 |
−0.130 |
0.115 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__21DPE |
28°C__14DPE |
−0.013 |
−0.136 |
0.109 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__28DPE |
28°C__14DPE |
−0.019 |
−0.141 |
0.103 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__42DPE |
28°C__14DPE |
0.078 |
−0.048 |
0.204 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
28°C__28DPE |
28°C__21DPE |
−0.068 |
−0.181 |
0.044 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
28°C__42DPE |
28°C__21DPE |
−0.063 |
−0.179 |
0.052 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__0DPE |
28°C__21DPE |
0.040 |
−0.057 |
0.138 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__14DPE |
28°C__21DPE |
−0.016 |
−0.128 |
0.097 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__21DPE |
28°C__21DPE |
−0.065 |
−0.177 |
0.048 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__28DPE |
28°C__21DPE |
−0.051 |
−0.167 |
0.065 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__42DPE |
28°C__21DPE |
−0.062 |
−0.175 |
0.050 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__0DPE |
28°C__21DPE |
0.014 |
−0.084 |
0.111 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__14DPE |
28°C__21DPE |
−0.036 |
−0.151 |
0.080 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__21DPE |
28°C__21DPE |
−0.041 |
−0.157 |
0.074 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__28DPE |
28°C__21DPE |
−0.047 |
−0.162 |
0.069 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__42DPE |
28°C__21DPE |
0.050 |
−0.070 |
0.169 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
28°C__42DPE |
28°C__28DPE |
0.005 |
−0.110 |
0.121 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__0DPE |
28°C__28DPE |
0.109 |
0.011 |
0.206 |
0.014 |
* |
| Distance |
Temp.DPE |
32°C__14DPE |
28°C__28DPE |
0.052 |
−0.060 |
0.165 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__21DPE |
28°C__28DPE |
0.004 |
−0.109 |
0.116 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__28DPE |
28°C__28DPE |
0.017 |
−0.098 |
0.133 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__42DPE |
28°C__28DPE |
0.006 |
−0.106 |
0.119 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__0DPE |
28°C__28DPE |
0.082 |
−0.015 |
0.180 |
0.206 |
ns |
| Distance |
Temp.DPE |
35°C__14DPE |
28°C__28DPE |
0.033 |
−0.083 |
0.148 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__21DPE |
28°C__28DPE |
0.027 |
−0.088 |
0.143 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__28DPE |
28°C__28DPE |
0.021 |
−0.094 |
0.137 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__42DPE |
28°C__28DPE |
0.118 |
−0.001 |
0.237 |
0.055 |
ns |
| Distance |
Temp.DPE |
32°C__0DPE |
28°C__42DPE |
0.104 |
0.003 |
0.205 |
0.038 |
* |
| Distance |
Temp.DPE |
32°C__14DPE |
28°C__42DPE |
0.047 |
−0.068 |
0.163 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__21DPE |
28°C__42DPE |
−0.001 |
−0.117 |
0.114 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__28DPE |
28°C__42DPE |
0.012 |
−0.106 |
0.131 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__42DPE |
28°C__42DPE |
0.001 |
−0.115 |
0.117 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__0DPE |
28°C__42DPE |
0.077 |
−0.024 |
0.178 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__14DPE |
28°C__42DPE |
0.028 |
−0.091 |
0.146 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__21DPE |
28°C__42DPE |
0.022 |
−0.097 |
0.141 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__28DPE |
28°C__42DPE |
0.016 |
−0.102 |
0.135 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__42DPE |
28°C__42DPE |
0.113 |
−0.009 |
0.235 |
0.104 |
ns |
| Distance |
Temp.DPE |
32°C__14DPE |
32°C__0DPE |
−0.056 |
−0.154 |
0.041 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__21DPE |
32°C__0DPE |
−0.105 |
−0.202 |
−0.007 |
0.022 |
* |
| Distance |
Temp.DPE |
32°C__28DPE |
32°C__0DPE |
−0.091 |
−0.192 |
0.010 |
0.125 |
ns |
| Distance |
Temp.DPE |
32°C__42DPE |
32°C__0DPE |
−0.103 |
−0.200 |
−0.005 |
0.029 |
* |
| Distance |
Temp.DPE |
35°C__0DPE |
32°C__0DPE |
−0.027 |
−0.106 |
0.053 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__14DPE |
32°C__0DPE |
−0.076 |
−0.177 |
0.025 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__21DPE |
32°C__0DPE |
−0.082 |
−0.183 |
0.019 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__28DPE |
32°C__0DPE |
−0.087 |
−0.188 |
0.014 |
0.175 |
ns |
| Distance |
Temp.DPE |
35°C__42DPE |
32°C__0DPE |
0.009 |
−0.096 |
0.115 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__21DPE |
32°C__14DPE |
−0.049 |
−0.161 |
0.064 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__28DPE |
32°C__14DPE |
−0.035 |
−0.151 |
0.081 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__42DPE |
32°C__14DPE |
−0.046 |
−0.159 |
0.066 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__0DPE |
32°C__14DPE |
0.030 |
−0.068 |
0.127 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__14DPE |
32°C__14DPE |
−0.020 |
−0.135 |
0.096 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__21DPE |
32°C__14DPE |
−0.025 |
−0.141 |
0.090 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__28DPE |
32°C__14DPE |
−0.031 |
−0.147 |
0.085 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__42DPE |
32°C__14DPE |
0.066 |
−0.054 |
0.185 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__28DPE |
32°C__21DPE |
0.014 |
−0.102 |
0.129 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__42DPE |
32°C__21DPE |
0.002 |
−0.110 |
0.115 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__0DPE |
32°C__21DPE |
0.078 |
−0.019 |
0.176 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__14DPE |
32°C__21DPE |
0.029 |
−0.087 |
0.145 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__21DPE |
32°C__21DPE |
0.023 |
−0.092 |
0.139 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__28DPE |
32°C__21DPE |
0.018 |
−0.098 |
0.133 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__42DPE |
32°C__21DPE |
0.114 |
−0.005 |
0.234 |
0.076 |
ns |
| Distance |
Temp.DPE |
32°C__42DPE |
32°C__28DPE |
−0.011 |
−0.127 |
0.104 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__0DPE |
32°C__28DPE |
0.065 |
−0.036 |
0.166 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__14DPE |
32°C__28DPE |
0.015 |
−0.103 |
0.134 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__21DPE |
32°C__28DPE |
0.010 |
−0.109 |
0.128 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__28DPE |
32°C__28DPE |
0.004 |
−0.115 |
0.123 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__42DPE |
32°C__28DPE |
0.101 |
−0.022 |
0.223 |
0.237 |
ns |
| Distance |
Temp.DPE |
35°C__0DPE |
32°C__42DPE |
0.076 |
−0.021 |
0.173 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__14DPE |
32°C__42DPE |
0.027 |
−0.089 |
0.142 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__21DPE |
32°C__42DPE |
0.021 |
−0.095 |
0.137 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__28DPE |
32°C__42DPE |
0.015 |
−0.100 |
0.131 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__42DPE |
32°C__42DPE |
0.112 |
−0.007 |
0.231 |
0.091 |
ns |
| Distance |
Temp.DPE |
35°C__14DPE |
35°C__0DPE |
−0.049 |
−0.150 |
0.052 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__21DPE |
35°C__0DPE |
−0.055 |
−0.156 |
0.046 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__28DPE |
35°C__0DPE |
−0.061 |
−0.162 |
0.040 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__42DPE |
35°C__0DPE |
0.036 |
−0.069 |
0.141 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__21DPE |
35°C__14DPE |
−0.006 |
−0.124 |
0.113 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__28DPE |
35°C__14DPE |
−0.011 |
−0.130 |
0.107 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__42DPE |
35°C__14DPE |
0.085 |
−0.037 |
0.208 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__28DPE |
35°C__21DPE |
−0.006 |
−0.124 |
0.113 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__42DPE |
35°C__21DPE |
0.091 |
−0.031 |
0.213 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__42DPE |
35°C__28DPE |
0.097 |
−0.026 |
0.219 |
≥0.25 |
ns |
| gunifrac |
| Distance |
Temp.DPE |
28°C__14DPE |
28°C__0DPE |
−0.005 |
−0.116 |
0.107 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
28°C__21DPE |
28°C__0DPE |
0.038 |
−0.065 |
0.141 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
28°C__28DPE |
28°C__0DPE |
−0.010 |
−0.113 |
0.093 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
28°C__42DPE |
28°C__0DPE |
−0.033 |
−0.140 |
0.073 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__0DPE |
28°C__0DPE |
0.067 |
−0.017 |
0.151 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__14DPE |
28°C__0DPE |
0.018 |
−0.085 |
0.121 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__21DPE |
28°C__0DPE |
−0.023 |
−0.126 |
0.080 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__28DPE |
28°C__0DPE |
−0.014 |
−0.121 |
0.093 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__42DPE |
28°C__0DPE |
−0.026 |
−0.129 |
0.077 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__0DPE |
28°C__0DPE |
0.029 |
−0.055 |
0.113 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__14DPE |
28°C__0DPE |
−0.015 |
−0.122 |
0.092 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__21DPE |
28°C__0DPE |
−0.020 |
−0.127 |
0.087 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__28DPE |
28°C__0DPE |
−0.065 |
−0.171 |
0.042 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__42DPE |
28°C__0DPE |
0.091 |
−0.020 |
0.202 |
0.245 |
ns |
| Distance |
Temp.DPE |
28°C__21DPE |
28°C__14DPE |
0.042 |
−0.084 |
0.168 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
28°C__28DPE |
28°C__14DPE |
−0.005 |
−0.131 |
0.121 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
28°C__42DPE |
28°C__14DPE |
−0.029 |
−0.158 |
0.100 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__0DPE |
28°C__14DPE |
0.072 |
−0.040 |
0.183 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__14DPE |
28°C__14DPE |
0.022 |
−0.104 |
0.148 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__21DPE |
28°C__14DPE |
−0.019 |
−0.145 |
0.107 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__28DPE |
28°C__14DPE |
−0.009 |
−0.138 |
0.120 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__42DPE |
28°C__14DPE |
−0.021 |
−0.147 |
0.105 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__0DPE |
28°C__14DPE |
0.033 |
−0.078 |
0.145 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__14DPE |
28°C__14DPE |
−0.010 |
−0.139 |
0.119 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__21DPE |
28°C__14DPE |
−0.015 |
−0.145 |
0.114 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__28DPE |
28°C__14DPE |
−0.060 |
−0.189 |
0.069 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__42DPE |
28°C__14DPE |
0.096 |
−0.037 |
0.229 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
28°C__28DPE |
28°C__21DPE |
−0.047 |
−0.166 |
0.072 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
28°C__42DPE |
28°C__21DPE |
−0.071 |
−0.193 |
0.051 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__0DPE |
28°C__21DPE |
0.029 |
−0.074 |
0.132 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__14DPE |
28°C__21DPE |
−0.020 |
−0.139 |
0.099 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__21DPE |
28°C__21DPE |
−0.061 |
−0.180 |
0.058 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__28DPE |
28°C__21DPE |
−0.051 |
−0.174 |
0.071 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__42DPE |
28°C__21DPE |
−0.063 |
−0.182 |
0.056 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__0DPE |
28°C__21DPE |
−0.009 |
−0.112 |
0.094 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__14DPE |
28°C__21DPE |
−0.052 |
−0.175 |
0.070 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__21DPE |
28°C__21DPE |
−0.058 |
−0.180 |
0.065 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__28DPE |
28°C__21DPE |
−0.102 |
−0.224 |
0.020 |
0.216 |
ns |
| Distance |
Temp.DPE |
35°C__42DPE |
28°C__21DPE |
0.054 |
−0.073 |
0.180 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
28°C__42DPE |
28°C__28DPE |
−0.024 |
−0.146 |
0.098 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__0DPE |
28°C__28DPE |
0.077 |
−0.026 |
0.180 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__14DPE |
28°C__28DPE |
0.027 |
−0.092 |
0.146 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__21DPE |
28°C__28DPE |
−0.014 |
−0.133 |
0.105 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__28DPE |
28°C__28DPE |
−0.004 |
−0.126 |
0.118 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__42DPE |
28°C__28DPE |
−0.016 |
−0.135 |
0.103 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__0DPE |
28°C__28DPE |
0.038 |
−0.065 |
0.142 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__14DPE |
28°C__28DPE |
−0.005 |
−0.127 |
0.117 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__21DPE |
28°C__28DPE |
−0.010 |
−0.132 |
0.112 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__28DPE |
28°C__28DPE |
−0.055 |
−0.177 |
0.067 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__42DPE |
28°C__28DPE |
0.101 |
−0.025 |
0.227 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__0DPE |
28°C__42DPE |
0.100 |
−0.006 |
0.207 |
0.089 |
ns |
| Distance |
Temp.DPE |
32°C__14DPE |
28°C__42DPE |
0.051 |
−0.071 |
0.173 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__21DPE |
28°C__42DPE |
0.010 |
−0.112 |
0.132 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__28DPE |
28°C__42DPE |
0.020 |
−0.106 |
0.145 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__42DPE |
28°C__42DPE |
0.008 |
−0.114 |
0.130 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__0DPE |
28°C__42DPE |
0.062 |
−0.045 |
0.169 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__14DPE |
28°C__42DPE |
0.019 |
−0.107 |
0.144 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__21DPE |
28°C__42DPE |
0.014 |
−0.112 |
0.139 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__28DPE |
28°C__42DPE |
−0.031 |
−0.157 |
0.094 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__42DPE |
28°C__42DPE |
0.125 |
−0.005 |
0.254 |
0.071 |
ns |
| Distance |
Temp.DPE |
32°C__14DPE |
32°C__0DPE |
−0.049 |
−0.152 |
0.054 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__21DPE |
32°C__0DPE |
−0.090 |
−0.193 |
0.013 |
0.155 |
ns |
| Distance |
Temp.DPE |
32°C__28DPE |
32°C__0DPE |
−0.081 |
−0.188 |
0.026 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__42DPE |
32°C__0DPE |
−0.093 |
−0.196 |
0.010 |
0.130 |
ns |
| Distance |
Temp.DPE |
35°C__0DPE |
32°C__0DPE |
−0.038 |
−0.122 |
0.046 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__14DPE |
32°C__0DPE |
−0.082 |
−0.189 |
0.025 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__21DPE |
32°C__0DPE |
−0.087 |
−0.194 |
0.020 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__28DPE |
32°C__0DPE |
−0.132 |
−0.238 |
−0.025 |
0.003 |
** |
| Distance |
Temp.DPE |
35°C__42DPE |
32°C__0DPE |
0.024 |
−0.087 |
0.135 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__21DPE |
32°C__14DPE |
−0.041 |
−0.160 |
0.078 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__28DPE |
32°C__14DPE |
−0.031 |
−0.154 |
0.091 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__42DPE |
32°C__14DPE |
−0.043 |
−0.162 |
0.076 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__0DPE |
32°C__14DPE |
0.011 |
−0.092 |
0.114 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__14DPE |
32°C__14DPE |
−0.032 |
−0.155 |
0.090 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__21DPE |
32°C__14DPE |
−0.037 |
−0.160 |
0.085 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__28DPE |
32°C__14DPE |
−0.082 |
−0.204 |
0.040 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__42DPE |
32°C__14DPE |
0.074 |
−0.053 |
0.200 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__28DPE |
32°C__21DPE |
0.010 |
−0.113 |
0.132 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__42DPE |
32°C__21DPE |
−0.002 |
−0.121 |
0.117 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__0DPE |
32°C__21DPE |
0.052 |
−0.051 |
0.155 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__14DPE |
32°C__21DPE |
0.009 |
−0.114 |
0.131 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__21DPE |
32°C__21DPE |
0.004 |
−0.119 |
0.126 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__28DPE |
32°C__21DPE |
−0.041 |
−0.163 |
0.081 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__42DPE |
32°C__21DPE |
0.115 |
−0.011 |
0.241 |
0.119 |
ns |
| Distance |
Temp.DPE |
32°C__42DPE |
32°C__28DPE |
−0.012 |
−0.134 |
0.110 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__0DPE |
32°C__28DPE |
0.043 |
−0.064 |
0.149 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__14DPE |
32°C__28DPE |
−0.001 |
−0.126 |
0.124 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__21DPE |
32°C__28DPE |
−0.006 |
−0.132 |
0.119 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__28DPE |
32°C__28DPE |
−0.051 |
−0.176 |
0.075 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__42DPE |
32°C__28DPE |
0.105 |
−0.024 |
0.234 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__0DPE |
32°C__42DPE |
0.054 |
−0.049 |
0.157 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__14DPE |
32°C__42DPE |
0.011 |
−0.111 |
0.133 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__21DPE |
32°C__42DPE |
0.006 |
−0.117 |
0.128 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__28DPE |
32°C__42DPE |
−0.039 |
−0.161 |
0.083 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__42DPE |
32°C__42DPE |
0.117 |
−0.009 |
0.243 |
0.102 |
ns |
| Distance |
Temp.DPE |
35°C__14DPE |
35°C__0DPE |
−0.044 |
−0.150 |
0.063 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__21DPE |
35°C__0DPE |
−0.049 |
−0.155 |
0.058 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__28DPE |
35°C__0DPE |
−0.093 |
−0.200 |
0.013 |
0.160 |
ns |
| Distance |
Temp.DPE |
35°C__42DPE |
35°C__0DPE |
0.062 |
−0.049 |
0.174 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__21DPE |
35°C__14DPE |
−0.005 |
−0.130 |
0.120 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__28DPE |
35°C__14DPE |
−0.050 |
−0.175 |
0.076 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__42DPE |
35°C__14DPE |
0.106 |
−0.023 |
0.235 |
0.244 |
ns |
| Distance |
Temp.DPE |
35°C__28DPE |
35°C__21DPE |
−0.045 |
−0.170 |
0.081 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__42DPE |
35°C__21DPE |
0.111 |
−0.018 |
0.240 |
0.180 |
ns |
| Distance |
Temp.DPE |
35°C__42DPE |
35°C__28DPE |
0.156 |
0.027 |
0.285 |
0.005 |
** |
3) Parasitic worm burden differs by water
temperature
Fig. 3 Histological sections stained with ### stain in zebrafish
exposed to Pseudocapillaria tomentosa examined at ### days post
exposure. (A) Nematodes in intestinal lumen. Arrow = ###. (B) Infection
outcome analysis of fish exposed to P. tomentosa (n = 89) by
temperature. Fish reared at 28°C and 32°C water temperatures had
significantly different infection burden to fish reared at 35°C water
temperature. Only one fish reared at 35°C was identified as being
positively infected by wet mount.
*3A)
3B)

4) Water temperature differentially stratifies gut
microbiome communities of parasite exposed fish
Fig. 4 Effects of parasite exposure and water temperature on gut
microbiomes of zebrafish. (A) Simpson’s Index of diversity shows that
gut microbiome diversity significantly differs between fish reared at
28°C water temperature to fish reared at 32°C and 35°C water
temperatures. (B) Capscale ordination based on the Bray-Curtis
dissimilarity of gut microbiome composition. The analysis shows that gut
microbiome composition significantly differs between parasite exposed
fish reared at different water temperatures. (C) Simpson’s Index of
diversity shows microbial gut diversity decreases with time from 0 days
post exposure (dpe) to 42 dpe in parasite exposed fish reared at 28°C
water temperature. (D) Capscale ordination of gut microbiome composition
based on the Canberra dissimilarity constrained on the main effects of
water temperature and time, and their interaction. The analysis shows
that shows that gut microbiome composition differs between parasite
exposed fish across time depending on water temperature. Ribbons and
ellipses indicate 95% confidence interval. Only statistically
significant relationships are shown. A “*” indicates statistical
significance below the “0.05” level. Black arrows indicate direction of
greatest change in the indicated covariates.
4A)

Tables
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GLM
| GLM Results |
| glm(Alpha.Score ~ Temperature); Exposed fish |
| term |
estimate |
std.error |
statistic |
p.value |
p.adj.sig |
| Shannon |
| (Intercept) |
−0.936 |
0.233 |
−4.021 |
<0.001 |
*** |
| Temperature32 |
0.909 |
0.301 |
3.017 |
0.003 |
** |
| Temperature35 |
0.516 |
0.296 |
1.741 |
0.085 |
ns |
| Simpson |
| (Intercept) |
−1.002 |
0.243 |
−4.130 |
<0.001 |
**** |
| Temperature32 |
1.057 |
0.312 |
3.384 |
0.001 |
** |
| Temperature35 |
0.772 |
0.306 |
2.525 |
0.013 |
* |
| Richness |
| (Intercept) |
−0.681 |
0.190 |
−3.583 |
<0.001 |
*** |
| Temperature32 |
0.703 |
0.251 |
2.800 |
0.006 |
** |
| Temperature35 |
0.242 |
0.247 |
0.978 |
≥0.25 |
ns |
ANOVA
| ANOVA of GLM |
| ANOVA(GLM(Alpha.Score ~ Temperature), type = 2); Exposed fish |
| term |
statistic |
df |
p.value |
sig |
| Shannon |
| Temperature |
9.440 |
2.000 |
0.009 |
** |
| Simpson |
| Temperature |
12.497 |
2.000 |
0.002 |
** |
| Richness |
| Temperature |
8.575 |
2.000 |
0.014 |
* |
Tukey
| Pairwise Tukey's HSD, p.adj: Dunnett |
| Tukey(Alpha.Score ~ Temperature); Exposed fish |
| term |
.y. |
group1 |
group2 |
estimate |
std.error |
statistic |
adj.p.value |
Variable |
| Shannon |
| Temperature |
Alpha.Score |
32 |
28 |
0.909 |
0.301 |
3.017 |
0.007 |
Temperature |
| Temperature |
Alpha.Score |
35 |
28 |
0.516 |
0.296 |
1.741 |
0.190 |
Temperature |
| Temperature |
Alpha.Score |
35 |
32 |
−0.393 |
0.265 |
−1.482 |
≥0.25 |
Temperature |
| Simpson |
| Temperature |
Alpha.Score |
32 |
28 |
1.057 |
0.312 |
3.384 |
0.002 |
Temperature |
| Temperature |
Alpha.Score |
35 |
28 |
0.772 |
0.306 |
2.525 |
0.031 |
Temperature |
| Temperature |
Alpha.Score |
35 |
32 |
−0.285 |
0.270 |
−1.054 |
≥0.25 |
Temperature |
| Richness |
| Temperature |
Alpha.Score |
32 |
28 |
0.703 |
0.251 |
2.800 |
0.014 |
Temperature |
| Temperature |
Alpha.Score |
35 |
28 |
0.242 |
0.247 |
0.978 |
≥0.25 |
Temperature |
| Temperature |
Alpha.Score |
35 |
32 |
−0.461 |
0.228 |
−2.028 |
0.105 |
Temperature |
4B)

Tables
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Capscale
| ADONIS2 |
| adonis2(Beta Distance ~ Temperature); Exposed fish |
| term |
df |
SumOfSqs |
R2 |
statistic |
p.value |
sig |
| bray |
| Temperature |
2.000 |
5.218 |
0.251 |
14.447 |
0.001 |
*** |
| Residual |
86.000 |
15.530 |
0.749 |
NA |
NA |
NA |
| Total |
88.000 |
20.748 |
1.000 |
NA |
NA |
NA |
| canberra |
| Temperature |
2.000 |
3.573 |
0.117 |
5.701 |
0.001 |
*** |
| Residual |
86.000 |
26.947 |
0.883 |
NA |
NA |
NA |
| Total |
88.000 |
30.520 |
1.000 |
NA |
NA |
NA |
| gunifrac |
| Temperature |
2.000 |
3.363 |
0.212 |
11.590 |
0.001 |
*** |
| Residual |
86.000 |
12.478 |
0.788 |
NA |
NA |
NA |
| Total |
88.000 |
15.841 |
1.000 |
NA |
NA |
NA |
Dispersion (ANOVA)
| ANOVA: Homogeneity of Dispersion |
| ANOVA(Beta Disperson ~ Temperature); Exposed fish |
| term |
df |
sumsq |
meansq |
statistic |
p.value |
sig |
| bray |
| Temperature |
2.000 |
0.364 |
0.182 |
8.708 |
<0.001 |
*** |
| Residual |
86.000 |
1.796 |
0.021 |
NA |
NA |
NA |
| canberra |
| Temperature |
2.000 |
0.314 |
0.157 |
17.419 |
<0.001 |
**** |
| Residual |
86.000 |
0.774 |
0.009 |
NA |
NA |
NA |
| gunifrac |
| Temperature |
2.000 |
0.310 |
0.155 |
13.567 |
<0.001 |
**** |
| Residual |
86.000 |
0.983 |
0.011 |
NA |
NA |
NA |
Dispersion (Tukey)
| Tukey: Homogeneity of Dispersion |
| Tukey(Beta Disperson ~ Temperature); Exposed fish |
| .y. |
term |
group1 |
group2 |
estimate |
conf.low |
conf.high |
adj.p.value |
sig |
| bray |
| Distance |
Temperature |
32 |
28 |
−0.043 |
−0.136 |
0.051 |
≥0.25 |
ns |
| Distance |
Temperature |
35 |
28 |
−0.150 |
−0.241 |
−0.059 |
<0.001 |
*** |
| Distance |
Temperature |
35 |
32 |
−0.108 |
−0.194 |
−0.021 |
0.011 |
* |
| canberra |
| Distance |
Temperature |
32 |
28 |
−0.110 |
−0.171 |
−0.049 |
<0.001 |
*** |
| Distance |
Temperature |
35 |
28 |
−0.144 |
−0.204 |
−0.085 |
<0.001 |
**** |
| Distance |
Temperature |
35 |
32 |
−0.034 |
−0.091 |
0.022 |
≥0.25 |
ns |
| gunifrac |
| Distance |
Temperature |
32 |
28 |
−0.056 |
−0.125 |
0.013 |
0.133 |
ns |
| Distance |
Temperature |
35 |
28 |
−0.143 |
−0.211 |
−0.076 |
<0.001 |
**** |
| Distance |
Temperature |
35 |
32 |
−0.087 |
−0.151 |
−0.023 |
0.005 |
** |
4C)

Tables
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GLM
| GLM Results |
| glm(Alpha.Score ~ Temperature*DPE); Exposed fish |
| term |
estimate |
std.error |
statistic |
p.value |
p.adj.sig |
| Shannon |
| (Intercept) |
−0.134 |
0.594 |
−0.226 |
≥0.25 |
ns |
| Temperature32 |
0.228 |
0.774 |
0.295 |
≥0.25 |
ns |
| Temperature35 |
−0.428 |
0.777 |
−0.551 |
≥0.25 |
ns |
| DPE |
−0.031 |
0.022 |
−1.425 |
0.158 |
ns |
| Temperature32:DPE |
0.026 |
0.028 |
0.917 |
≥0.25 |
ns |
| Temperature35:DPE |
0.036 |
0.028 |
1.300 |
0.197 |
ns |
| Simpson |
| (Intercept) |
−0.282 |
0.617 |
−0.456 |
≥0.25 |
ns |
| Temperature32 |
0.336 |
0.801 |
0.420 |
≥0.25 |
ns |
| Temperature35 |
−0.172 |
0.799 |
−0.215 |
≥0.25 |
ns |
| DPE |
−0.028 |
0.022 |
−1.234 |
0.221 |
ns |
| Temperature32:DPE |
0.028 |
0.029 |
0.946 |
≥0.25 |
ns |
| Temperature35:DPE |
0.036 |
0.029 |
1.261 |
0.211 |
ns |
| Richness |
| (Intercept) |
0.316 |
0.480 |
0.657 |
≥0.25 |
ns |
| Temperature32 |
−0.034 |
0.636 |
−0.054 |
≥0.25 |
ns |
| Temperature35 |
−0.596 |
0.638 |
−0.934 |
≥0.25 |
ns |
| DPE |
−0.038 |
0.017 |
−2.191 |
0.031 |
* |
| Temperature32:DPE |
0.028 |
0.023 |
1.199 |
0.234 |
ns |
| Temperature35:DPE |
0.032 |
0.023 |
1.405 |
0.164 |
ns |
ANOVA
| ANOVA of GLM |
| ANOVA(GLM(Alpha.Score ~ Temperature*Time), type = 2); Exposed fish |
| term |
statistic |
df |
p.value |
sig |
| Shannon |
| Temperature |
8.789 |
2.000 |
0.012 |
* |
| DPE |
0.523 |
1.000 |
≥0.25 |
ns |
| Temperature:DPE |
1.759 |
2.000 |
≥0.25 |
ns |
| Simpson |
| Temperature |
11.871 |
2.000 |
0.003 |
** |
| DPE |
0.120 |
1.000 |
≥0.25 |
ns |
| Temperature:DPE |
1.684 |
2.000 |
≥0.25 |
ns |
| Richness |
| Temperature |
7.911 |
2.000 |
0.019 |
* |
| DPE |
3.435 |
1.000 |
0.064 |
ns |
| Temperature:DPE |
2.255 |
2.000 |
≥0.25 |
ns |
Tukey
| Pairwise Tukey's HSD, p.adj: Dunnett |
| Tukey(Alpha.Score ~ Temperature*Time); Exposed fish |
| Temperature |
.y. |
term |
group1 |
group2 |
estimate |
std.error |
statistic |
adj.p.value |
Variable |
Group |
| Shannon |
| 28 |
Alpha.Score |
DPE |
21 |
14 |
−3.311 |
1.822 |
−1.817 |
0.246 |
DPE |
28 |
| 28 |
Alpha.Score |
DPE |
28 |
14 |
−2.027 |
1.023 |
−1.982 |
0.178 |
DPE |
28 |
| 28 |
Alpha.Score |
DPE |
42 |
14 |
−1.024 |
0.705 |
−1.452 |
≥0.25 |
DPE |
28 |
| 28 |
Alpha.Score |
DPE |
28 |
21 |
1.284 |
1.982 |
0.648 |
≥0.25 |
DPE |
28 |
| 28 |
Alpha.Score |
DPE |
42 |
21 |
2.287 |
1.838 |
1.245 |
≥0.25 |
DPE |
28 |
| 28 |
Alpha.Score |
DPE |
42 |
28 |
1.004 |
1.050 |
0.956 |
≥0.25 |
DPE |
28 |
| 32 |
Alpha.Score |
DPE |
21 |
14 |
−0.536 |
0.537 |
−0.999 |
≥0.25 |
DPE |
32 |
| 32 |
Alpha.Score |
DPE |
28 |
14 |
0.056 |
0.558 |
0.099 |
≥0.25 |
DPE |
32 |
| 32 |
Alpha.Score |
DPE |
42 |
14 |
−0.271 |
0.532 |
−0.509 |
≥0.25 |
DPE |
32 |
| 32 |
Alpha.Score |
DPE |
28 |
21 |
0.592 |
0.606 |
0.977 |
≥0.25 |
DPE |
32 |
| 32 |
Alpha.Score |
DPE |
42 |
21 |
0.266 |
0.581 |
0.457 |
≥0.25 |
DPE |
32 |
| 32 |
Alpha.Score |
DPE |
42 |
28 |
−0.326 |
0.601 |
−0.543 |
≥0.25 |
DPE |
32 |
| 35 |
Alpha.Score |
DPE |
21 |
14 |
−0.246 |
0.270 |
−0.909 |
≥0.25 |
DPE |
35 |
| 35 |
Alpha.Score |
DPE |
28 |
14 |
−0.292 |
0.271 |
−1.075 |
≥0.25 |
DPE |
35 |
| 35 |
Alpha.Score |
DPE |
42 |
14 |
0.097 |
0.258 |
0.377 |
≥0.25 |
DPE |
35 |
| 35 |
Alpha.Score |
DPE |
28 |
21 |
−0.046 |
0.283 |
−0.162 |
≥0.25 |
DPE |
35 |
| 35 |
Alpha.Score |
DPE |
42 |
21 |
0.343 |
0.270 |
1.273 |
≥0.25 |
DPE |
35 |
| 35 |
Alpha.Score |
DPE |
42 |
28 |
0.389 |
0.271 |
1.438 |
≥0.25 |
DPE |
35 |
| Simpson |
| 28 |
Alpha.Score |
DPE |
21 |
14 |
−3.141 |
1.820 |
−1.725 |
≥0.25 |
DPE |
28 |
| 28 |
Alpha.Score |
DPE |
28 |
14 |
−1.994 |
1.072 |
−1.861 |
0.226 |
DPE |
28 |
| 28 |
Alpha.Score |
DPE |
42 |
14 |
−0.925 |
0.721 |
−1.283 |
≥0.25 |
DPE |
28 |
| 28 |
Alpha.Score |
DPE |
28 |
21 |
1.147 |
2.001 |
0.573 |
≥0.25 |
DPE |
28 |
| 28 |
Alpha.Score |
DPE |
42 |
21 |
2.215 |
1.837 |
1.206 |
≥0.25 |
DPE |
28 |
| 28 |
Alpha.Score |
DPE |
42 |
28 |
1.069 |
1.100 |
0.971 |
≥0.25 |
DPE |
28 |
| 32 |
Alpha.Score |
DPE |
21 |
14 |
−0.416 |
0.550 |
−0.756 |
≥0.25 |
DPE |
32 |
| 32 |
Alpha.Score |
DPE |
28 |
14 |
0.275 |
0.580 |
0.474 |
≥0.25 |
DPE |
32 |
| 32 |
Alpha.Score |
DPE |
42 |
14 |
−0.138 |
0.546 |
−0.252 |
≥0.25 |
DPE |
32 |
| 32 |
Alpha.Score |
DPE |
28 |
21 |
0.691 |
0.626 |
1.103 |
≥0.25 |
DPE |
32 |
| 32 |
Alpha.Score |
DPE |
42 |
21 |
0.278 |
0.595 |
0.467 |
≥0.25 |
DPE |
32 |
| 32 |
Alpha.Score |
DPE |
42 |
28 |
−0.413 |
0.623 |
−0.662 |
≥0.25 |
DPE |
32 |
| 35 |
Alpha.Score |
DPE |
21 |
14 |
−0.374 |
0.297 |
−1.258 |
≥0.25 |
DPE |
35 |
| 35 |
Alpha.Score |
DPE |
28 |
14 |
−0.285 |
0.296 |
−0.966 |
≥0.25 |
DPE |
35 |
| 35 |
Alpha.Score |
DPE |
42 |
14 |
0.142 |
0.284 |
0.502 |
≥0.25 |
DPE |
35 |
| 35 |
Alpha.Score |
DPE |
28 |
21 |
0.088 |
0.308 |
0.287 |
≥0.25 |
DPE |
35 |
| 35 |
Alpha.Score |
DPE |
42 |
21 |
0.516 |
0.297 |
1.738 |
≥0.25 |
DPE |
35 |
| 35 |
Alpha.Score |
DPE |
42 |
28 |
0.428 |
0.295 |
1.448 |
≥0.25 |
DPE |
35 |
| Richness |
| 28 |
Alpha.Score |
DPE |
21 |
14 |
−2.219 |
1.016 |
−2.185 |
0.123 |
DPE |
28 |
| 28 |
Alpha.Score |
DPE |
28 |
14 |
−1.585 |
0.795 |
−1.993 |
0.184 |
DPE |
28 |
| 28 |
Alpha.Score |
DPE |
42 |
14 |
−1.239 |
0.650 |
−1.907 |
0.218 |
DPE |
28 |
| 28 |
Alpha.Score |
DPE |
28 |
21 |
0.634 |
1.133 |
0.560 |
≥0.25 |
DPE |
28 |
| 28 |
Alpha.Score |
DPE |
42 |
21 |
0.980 |
1.036 |
0.946 |
≥0.25 |
DPE |
28 |
| 28 |
Alpha.Score |
DPE |
42 |
28 |
0.346 |
0.821 |
0.421 |
≥0.25 |
DPE |
28 |
| 32 |
Alpha.Score |
DPE |
21 |
14 |
−0.561 |
0.412 |
−1.363 |
≥0.25 |
DPE |
32 |
| 32 |
Alpha.Score |
DPE |
28 |
14 |
−0.470 |
0.430 |
−1.091 |
≥0.25 |
DPE |
32 |
| 32 |
Alpha.Score |
DPE |
42 |
14 |
−0.357 |
0.410 |
−0.871 |
≥0.25 |
DPE |
32 |
| 32 |
Alpha.Score |
DPE |
28 |
21 |
0.092 |
0.462 |
0.199 |
≥0.25 |
DPE |
32 |
| 32 |
Alpha.Score |
DPE |
42 |
21 |
0.204 |
0.444 |
0.460 |
≥0.25 |
DPE |
32 |
| 32 |
Alpha.Score |
DPE |
42 |
28 |
0.112 |
0.461 |
0.243 |
≥0.25 |
DPE |
32 |
| 35 |
Alpha.Score |
DPE |
21 |
14 |
−0.338 |
0.242 |
−1.396 |
≥0.25 |
DPE |
35 |
| 35 |
Alpha.Score |
DPE |
28 |
14 |
−0.329 |
0.242 |
−1.361 |
≥0.25 |
DPE |
35 |
| 35 |
Alpha.Score |
DPE |
42 |
14 |
−0.233 |
0.233 |
−1.000 |
≥0.25 |
DPE |
35 |
| 35 |
Alpha.Score |
DPE |
28 |
21 |
0.009 |
0.252 |
0.035 |
≥0.25 |
DPE |
35 |
| 35 |
Alpha.Score |
DPE |
42 |
21 |
0.105 |
0.244 |
0.430 |
≥0.25 |
DPE |
35 |
| 35 |
Alpha.Score |
DPE |
42 |
28 |
0.096 |
0.244 |
0.394 |
≥0.25 |
DPE |
35 |
4D)

Tables
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Capscale
| ADONIS2 |
| adonis2(Beta Distance ~ Temperature*DPE); Exposed fish |
| term |
df |
SumOfSqs |
R2 |
statistic |
p.value |
sig |
| bray |
| Temperature |
2.000 |
5.218 |
0.251 |
14.843 |
0.001 |
*** |
| DPE |
1.000 |
0.394 |
0.019 |
2.241 |
0.040 |
* |
| Temperature:DPE |
2.000 |
0.548 |
0.026 |
1.558 |
0.110 |
ns |
| Residual |
83.000 |
14.589 |
0.703 |
NA |
NA |
NA |
| Total |
88.000 |
20.748 |
1.000 |
NA |
NA |
NA |
| canberra |
| Temperature |
2.000 |
3.573 |
0.117 |
5.847 |
0.001 |
*** |
| DPE |
1.000 |
0.674 |
0.022 |
2.207 |
0.004 |
** |
| Temperature:DPE |
2.000 |
0.913 |
0.030 |
1.494 |
0.015 |
* |
| Residual |
83.000 |
25.360 |
0.831 |
NA |
NA |
NA |
| Total |
88.000 |
30.520 |
1.000 |
NA |
NA |
NA |
| gunifrac |
| Temperature |
2.000 |
3.363 |
0.212 |
11.847 |
0.001 |
*** |
| DPE |
1.000 |
0.261 |
0.016 |
1.837 |
0.102 |
ns |
| Temperature:DPE |
2.000 |
0.436 |
0.028 |
1.535 |
0.134 |
ns |
| Residual |
83.000 |
11.781 |
0.744 |
NA |
NA |
NA |
| Total |
88.000 |
15.841 |
1.000 |
NA |
NA |
NA |
Dispersion (ANOVA)
| ANOVA: Homogeneity of Dispersion |
| ANOVA(Beta Disperson ~ Temperature); Exposed fish |
| term |
df |
sumsq |
meansq |
statistic |
p.value |
sig |
| bray |
| Temp.DPE |
11.000 |
0.624 |
0.057 |
3.261 |
0.001 |
** |
| Residual |
77.000 |
1.339 |
0.017 |
NA |
NA |
NA |
| canberra |
| Temp.DPE |
11.000 |
0.316 |
0.029 |
2.748 |
0.005 |
** |
| Residual |
77.000 |
0.805 |
0.010 |
NA |
NA |
NA |
| gunifrac |
| Temp.DPE |
11.000 |
0.420 |
0.038 |
3.232 |
0.001 |
** |
| Residual |
77.000 |
0.909 |
0.012 |
NA |
NA |
NA |
Dispersion (Tukey)
| Tukey: Homogeneity of Dispersion |
| Tukey(Beta Disperson ~ Temperature*DPE); Exposed fish |
| .y. |
term |
group1 |
group2 |
estimate |
conf.low |
conf.high |
adj.p.value |
sig |
| bray |
| Distance |
Temp.DPE |
28°C__21DPE |
28°C__14DPE |
−0.213 |
−0.485 |
0.059 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
28°C__28DPE |
28°C__14DPE |
−0.258 |
−0.512 |
−0.005 |
0.042 |
* |
| Distance |
Temp.DPE |
28°C__42DPE |
28°C__14DPE |
−0.063 |
−0.285 |
0.160 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__14DPE |
28°C__14DPE |
−0.059 |
−0.270 |
0.152 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__21DPE |
28°C__14DPE |
−0.089 |
−0.319 |
0.141 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__28DPE |
28°C__14DPE |
−0.123 |
−0.363 |
0.117 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__42DPE |
28°C__14DPE |
−0.142 |
−0.372 |
0.088 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__14DPE |
28°C__14DPE |
−0.202 |
−0.418 |
0.014 |
0.090 |
ns |
| Distance |
Temp.DPE |
35°C__21DPE |
28°C__14DPE |
−0.170 |
−0.393 |
0.052 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__28DPE |
28°C__14DPE |
−0.289 |
−0.511 |
−0.067 |
0.002 |
** |
| Distance |
Temp.DPE |
35°C__42DPE |
28°C__14DPE |
−0.192 |
−0.408 |
0.024 |
0.130 |
ns |
| Distance |
Temp.DPE |
28°C__28DPE |
28°C__21DPE |
−0.046 |
−0.344 |
0.253 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
28°C__42DPE |
28°C__21DPE |
0.150 |
−0.122 |
0.422 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__14DPE |
28°C__21DPE |
0.153 |
−0.110 |
0.416 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__21DPE |
28°C__21DPE |
0.124 |
−0.154 |
0.403 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__28DPE |
28°C__21DPE |
0.090 |
−0.197 |
0.377 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__42DPE |
28°C__21DPE |
0.071 |
−0.208 |
0.350 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__14DPE |
28°C__21DPE |
0.011 |
−0.256 |
0.278 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__21DPE |
28°C__21DPE |
0.042 |
−0.230 |
0.315 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__28DPE |
28°C__21DPE |
−0.077 |
−0.349 |
0.196 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__42DPE |
28°C__21DPE |
0.021 |
−0.246 |
0.288 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
28°C__42DPE |
28°C__28DPE |
0.196 |
−0.058 |
0.449 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__14DPE |
28°C__28DPE |
0.199 |
−0.044 |
0.443 |
0.220 |
ns |
| Distance |
Temp.DPE |
32°C__21DPE |
28°C__28DPE |
0.170 |
−0.091 |
0.430 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__28DPE |
28°C__28DPE |
0.136 |
−0.133 |
0.405 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__42DPE |
28°C__28DPE |
0.117 |
−0.144 |
0.377 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__14DPE |
28°C__28DPE |
0.057 |
−0.191 |
0.305 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__21DPE |
28°C__28DPE |
0.088 |
−0.165 |
0.341 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__28DPE |
28°C__28DPE |
−0.031 |
−0.284 |
0.222 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__42DPE |
28°C__28DPE |
0.066 |
−0.182 |
0.314 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__14DPE |
28°C__42DPE |
0.003 |
−0.207 |
0.214 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__21DPE |
28°C__42DPE |
−0.026 |
−0.256 |
0.204 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__28DPE |
28°C__42DPE |
−0.060 |
−0.300 |
0.180 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__42DPE |
28°C__42DPE |
−0.079 |
−0.309 |
0.151 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__14DPE |
28°C__42DPE |
−0.139 |
−0.355 |
0.077 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__21DPE |
28°C__42DPE |
−0.108 |
−0.330 |
0.115 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__28DPE |
28°C__42DPE |
−0.227 |
−0.449 |
−0.004 |
0.042 |
* |
| Distance |
Temp.DPE |
35°C__42DPE |
28°C__42DPE |
−0.129 |
−0.345 |
0.087 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__21DPE |
32°C__14DPE |
−0.029 |
−0.248 |
0.190 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__28DPE |
32°C__14DPE |
−0.063 |
−0.293 |
0.166 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__42DPE |
32°C__14DPE |
−0.082 |
−0.301 |
0.137 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__14DPE |
32°C__14DPE |
−0.142 |
−0.347 |
0.062 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__21DPE |
32°C__14DPE |
−0.111 |
−0.322 |
0.100 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__28DPE |
32°C__14DPE |
−0.230 |
−0.441 |
−0.019 |
0.021 |
* |
| Distance |
Temp.DPE |
35°C__42DPE |
32°C__14DPE |
−0.133 |
−0.337 |
0.072 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__28DPE |
32°C__21DPE |
−0.034 |
−0.281 |
0.213 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__42DPE |
32°C__21DPE |
−0.053 |
−0.291 |
0.185 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__14DPE |
32°C__21DPE |
−0.113 |
−0.337 |
0.111 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__21DPE |
32°C__21DPE |
−0.082 |
−0.312 |
0.148 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__28DPE |
32°C__21DPE |
−0.201 |
−0.431 |
0.029 |
0.148 |
ns |
| Distance |
Temp.DPE |
35°C__42DPE |
32°C__21DPE |
−0.103 |
−0.327 |
0.121 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__42DPE |
32°C__28DPE |
−0.019 |
−0.266 |
0.228 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__14DPE |
32°C__28DPE |
−0.079 |
−0.313 |
0.155 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__21DPE |
32°C__28DPE |
−0.048 |
−0.288 |
0.192 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__28DPE |
32°C__28DPE |
−0.167 |
−0.407 |
0.073 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__42DPE |
32°C__28DPE |
−0.069 |
−0.304 |
0.165 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__14DPE |
32°C__42DPE |
−0.060 |
−0.284 |
0.164 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__21DPE |
32°C__42DPE |
−0.029 |
−0.259 |
0.201 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__28DPE |
32°C__42DPE |
−0.148 |
−0.378 |
0.082 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__42DPE |
32°C__42DPE |
−0.050 |
−0.274 |
0.174 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__21DPE |
35°C__14DPE |
0.031 |
−0.185 |
0.247 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__28DPE |
35°C__14DPE |
−0.088 |
−0.304 |
0.128 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__42DPE |
35°C__14DPE |
0.010 |
−0.200 |
0.219 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__28DPE |
35°C__21DPE |
−0.119 |
−0.341 |
0.103 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__42DPE |
35°C__21DPE |
−0.022 |
−0.238 |
0.194 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__42DPE |
35°C__28DPE |
0.097 |
−0.119 |
0.313 |
≥0.25 |
ns |
| canberra |
| Distance |
Temp.DPE |
28°C__21DPE |
28°C__14DPE |
−0.004 |
−0.215 |
0.207 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
28°C__28DPE |
28°C__14DPE |
0.040 |
−0.156 |
0.237 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
28°C__42DPE |
28°C__14DPE |
0.059 |
−0.113 |
0.232 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__14DPE |
28°C__14DPE |
−0.043 |
−0.206 |
0.121 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__21DPE |
28°C__14DPE |
−0.044 |
−0.223 |
0.134 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__28DPE |
28°C__14DPE |
−0.102 |
−0.288 |
0.084 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__42DPE |
28°C__14DPE |
−0.084 |
−0.262 |
0.095 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__14DPE |
28°C__14DPE |
−0.097 |
−0.265 |
0.070 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__21DPE |
28°C__14DPE |
−0.097 |
−0.269 |
0.075 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__28DPE |
28°C__14DPE |
−0.142 |
−0.314 |
0.031 |
0.215 |
ns |
| Distance |
Temp.DPE |
35°C__42DPE |
28°C__14DPE |
−0.103 |
−0.271 |
0.064 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
28°C__28DPE |
28°C__21DPE |
0.044 |
−0.187 |
0.276 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
28°C__42DPE |
28°C__21DPE |
0.064 |
−0.148 |
0.275 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__14DPE |
28°C__21DPE |
−0.039 |
−0.243 |
0.165 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__21DPE |
28°C__21DPE |
−0.040 |
−0.256 |
0.176 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__28DPE |
28°C__21DPE |
−0.097 |
−0.320 |
0.125 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__42DPE |
28°C__21DPE |
−0.079 |
−0.295 |
0.137 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__14DPE |
28°C__21DPE |
−0.093 |
−0.300 |
0.114 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__21DPE |
28°C__21DPE |
−0.093 |
−0.304 |
0.118 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__28DPE |
28°C__21DPE |
−0.137 |
−0.348 |
0.074 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__42DPE |
28°C__21DPE |
−0.099 |
−0.306 |
0.108 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
28°C__42DPE |
28°C__28DPE |
0.019 |
−0.177 |
0.216 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__14DPE |
28°C__28DPE |
−0.083 |
−0.272 |
0.106 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__21DPE |
28°C__28DPE |
−0.085 |
−0.286 |
0.117 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__28DPE |
28°C__28DPE |
−0.142 |
−0.351 |
0.067 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__42DPE |
28°C__28DPE |
−0.124 |
−0.326 |
0.078 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__14DPE |
28°C__28DPE |
−0.137 |
−0.329 |
0.055 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__21DPE |
28°C__28DPE |
−0.137 |
−0.334 |
0.059 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__28DPE |
28°C__28DPE |
−0.182 |
−0.378 |
0.015 |
0.097 |
ns |
| Distance |
Temp.DPE |
35°C__42DPE |
28°C__28DPE |
−0.144 |
−0.336 |
0.049 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__14DPE |
28°C__42DPE |
−0.102 |
−0.266 |
0.061 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__21DPE |
28°C__42DPE |
−0.104 |
−0.282 |
0.075 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__28DPE |
28°C__42DPE |
−0.161 |
−0.347 |
0.025 |
0.157 |
ns |
| Distance |
Temp.DPE |
32°C__42DPE |
28°C__42DPE |
−0.143 |
−0.321 |
0.036 |
0.247 |
ns |
| Distance |
Temp.DPE |
35°C__14DPE |
28°C__42DPE |
−0.156 |
−0.324 |
0.011 |
0.091 |
ns |
| Distance |
Temp.DPE |
35°C__21DPE |
28°C__42DPE |
−0.156 |
−0.329 |
0.016 |
0.112 |
ns |
| Distance |
Temp.DPE |
35°C__28DPE |
28°C__42DPE |
−0.201 |
−0.373 |
−0.028 |
0.010 |
** |
| Distance |
Temp.DPE |
35°C__42DPE |
28°C__42DPE |
−0.163 |
−0.330 |
0.005 |
0.065 |
ns |
| Distance |
Temp.DPE |
32°C__21DPE |
32°C__14DPE |
−0.001 |
−0.171 |
0.168 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__28DPE |
32°C__14DPE |
−0.059 |
−0.237 |
0.119 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__42DPE |
32°C__14DPE |
−0.041 |
−0.211 |
0.129 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__14DPE |
32°C__14DPE |
−0.054 |
−0.212 |
0.104 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__21DPE |
32°C__14DPE |
−0.054 |
−0.218 |
0.109 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__28DPE |
32°C__14DPE |
−0.099 |
−0.262 |
0.065 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__42DPE |
32°C__14DPE |
−0.060 |
−0.219 |
0.098 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__28DPE |
32°C__21DPE |
−0.057 |
−0.249 |
0.134 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__42DPE |
32°C__21DPE |
−0.039 |
−0.224 |
0.145 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__14DPE |
32°C__21DPE |
−0.053 |
−0.226 |
0.121 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__21DPE |
32°C__21DPE |
−0.053 |
−0.231 |
0.126 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__28DPE |
32°C__21DPE |
−0.097 |
−0.276 |
0.081 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__42DPE |
32°C__21DPE |
−0.059 |
−0.233 |
0.115 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__42DPE |
32°C__28DPE |
0.018 |
−0.174 |
0.210 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__14DPE |
32°C__28DPE |
0.005 |
−0.177 |
0.186 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__21DPE |
32°C__28DPE |
0.005 |
−0.182 |
0.191 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__28DPE |
32°C__28DPE |
−0.040 |
−0.226 |
0.146 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__42DPE |
32°C__28DPE |
−0.002 |
−0.183 |
0.180 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__14DPE |
32°C__42DPE |
−0.013 |
−0.187 |
0.160 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__21DPE |
32°C__42DPE |
−0.014 |
−0.192 |
0.165 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__28DPE |
32°C__42DPE |
−0.058 |
−0.236 |
0.120 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__42DPE |
32°C__42DPE |
−0.020 |
−0.194 |
0.154 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__21DPE |
35°C__14DPE |
0.000 |
−0.168 |
0.167 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__28DPE |
35°C__14DPE |
−0.044 |
−0.212 |
0.123 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__42DPE |
35°C__14DPE |
−0.006 |
−0.169 |
0.156 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__28DPE |
35°C__21DPE |
−0.044 |
−0.217 |
0.128 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__42DPE |
35°C__21DPE |
−0.006 |
−0.174 |
0.161 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__42DPE |
35°C__28DPE |
0.038 |
−0.129 |
0.206 |
≥0.25 |
ns |
| gunifrac |
| Distance |
Temp.DPE |
28°C__21DPE |
28°C__14DPE |
−0.191 |
−0.415 |
0.033 |
0.173 |
ns |
| Distance |
Temp.DPE |
28°C__28DPE |
28°C__14DPE |
−0.061 |
−0.270 |
0.147 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
28°C__42DPE |
28°C__14DPE |
0.018 |
−0.165 |
0.201 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__14DPE |
28°C__14DPE |
−0.024 |
−0.197 |
0.150 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__21DPE |
28°C__14DPE |
−0.027 |
−0.216 |
0.163 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__28DPE |
28°C__14DPE |
−0.100 |
−0.298 |
0.098 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__42DPE |
28°C__14DPE |
−0.069 |
−0.259 |
0.120 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__14DPE |
28°C__14DPE |
−0.131 |
−0.309 |
0.047 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__21DPE |
28°C__14DPE |
−0.137 |
−0.320 |
0.046 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__28DPE |
28°C__14DPE |
−0.198 |
−0.382 |
−0.015 |
0.022 |
* |
| Distance |
Temp.DPE |
35°C__42DPE |
28°C__14DPE |
−0.134 |
−0.312 |
0.044 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
28°C__28DPE |
28°C__21DPE |
0.130 |
−0.116 |
0.375 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
28°C__42DPE |
28°C__21DPE |
0.209 |
−0.015 |
0.433 |
0.092 |
ns |
| Distance |
Temp.DPE |
32°C__14DPE |
28°C__21DPE |
0.167 |
−0.049 |
0.384 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__21DPE |
28°C__21DPE |
0.164 |
−0.065 |
0.394 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__28DPE |
28°C__21DPE |
0.091 |
−0.146 |
0.327 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__42DPE |
28°C__21DPE |
0.122 |
−0.108 |
0.351 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__14DPE |
28°C__21DPE |
0.060 |
−0.161 |
0.280 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__21DPE |
28°C__21DPE |
0.054 |
−0.170 |
0.278 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__28DPE |
28°C__21DPE |
−0.007 |
−0.232 |
0.217 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__42DPE |
28°C__21DPE |
0.057 |
−0.163 |
0.277 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
28°C__42DPE |
28°C__28DPE |
0.079 |
−0.130 |
0.288 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__14DPE |
28°C__28DPE |
0.038 |
−0.163 |
0.238 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__21DPE |
28°C__28DPE |
0.035 |
−0.180 |
0.249 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__28DPE |
28°C__28DPE |
−0.039 |
−0.260 |
0.183 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__42DPE |
28°C__28DPE |
−0.008 |
−0.222 |
0.206 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__14DPE |
28°C__28DPE |
−0.070 |
−0.274 |
0.134 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__21DPE |
28°C__28DPE |
−0.075 |
−0.284 |
0.133 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__28DPE |
28°C__28DPE |
−0.137 |
−0.346 |
0.072 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__42DPE |
28°C__28DPE |
−0.072 |
−0.277 |
0.132 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__14DPE |
28°C__42DPE |
−0.041 |
−0.215 |
0.132 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__21DPE |
28°C__42DPE |
−0.045 |
−0.234 |
0.145 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__28DPE |
28°C__42DPE |
−0.118 |
−0.316 |
0.080 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__42DPE |
28°C__42DPE |
−0.087 |
−0.277 |
0.102 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__14DPE |
28°C__42DPE |
−0.149 |
−0.327 |
0.029 |
0.190 |
ns |
| Distance |
Temp.DPE |
35°C__21DPE |
28°C__42DPE |
−0.155 |
−0.338 |
0.029 |
0.182 |
ns |
| Distance |
Temp.DPE |
35°C__28DPE |
28°C__42DPE |
−0.216 |
−0.399 |
−0.033 |
0.008 |
** |
| Distance |
Temp.DPE |
35°C__42DPE |
28°C__42DPE |
−0.151 |
−0.329 |
0.026 |
0.173 |
ns |
| Distance |
Temp.DPE |
32°C__21DPE |
32°C__14DPE |
−0.003 |
−0.184 |
0.177 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__28DPE |
32°C__14DPE |
−0.076 |
−0.266 |
0.113 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__42DPE |
32°C__14DPE |
−0.046 |
−0.226 |
0.135 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__14DPE |
32°C__14DPE |
−0.108 |
−0.276 |
0.060 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__21DPE |
32°C__14DPE |
−0.113 |
−0.287 |
0.061 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__28DPE |
32°C__14DPE |
−0.175 |
−0.348 |
−0.001 |
0.047 |
* |
| Distance |
Temp.DPE |
35°C__42DPE |
32°C__14DPE |
−0.110 |
−0.278 |
0.058 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__28DPE |
32°C__21DPE |
−0.073 |
−0.277 |
0.130 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__42DPE |
32°C__21DPE |
−0.043 |
−0.238 |
0.153 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__14DPE |
32°C__21DPE |
−0.105 |
−0.289 |
0.080 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__21DPE |
32°C__21DPE |
−0.110 |
−0.300 |
0.079 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__28DPE |
32°C__21DPE |
−0.172 |
−0.361 |
0.018 |
0.113 |
ns |
| Distance |
Temp.DPE |
35°C__42DPE |
32°C__21DPE |
−0.107 |
−0.292 |
0.078 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
32°C__42DPE |
32°C__28DPE |
0.031 |
−0.173 |
0.234 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__14DPE |
32°C__28DPE |
−0.031 |
−0.224 |
0.162 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__21DPE |
32°C__28DPE |
−0.037 |
−0.234 |
0.161 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__28DPE |
32°C__28DPE |
−0.098 |
−0.296 |
0.099 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__42DPE |
32°C__28DPE |
−0.034 |
−0.227 |
0.159 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__14DPE |
32°C__42DPE |
−0.062 |
−0.247 |
0.122 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__21DPE |
32°C__42DPE |
−0.067 |
−0.257 |
0.122 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__28DPE |
32°C__42DPE |
−0.129 |
−0.319 |
0.060 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__42DPE |
32°C__42DPE |
−0.064 |
−0.249 |
0.120 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__21DPE |
35°C__14DPE |
−0.005 |
−0.183 |
0.173 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__28DPE |
35°C__14DPE |
−0.067 |
−0.245 |
0.111 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__42DPE |
35°C__14DPE |
−0.002 |
−0.175 |
0.170 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__28DPE |
35°C__21DPE |
−0.062 |
−0.245 |
0.121 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__42DPE |
35°C__21DPE |
0.003 |
−0.175 |
0.181 |
≥0.25 |
ns |
| Distance |
Temp.DPE |
35°C__42DPE |
35°C__28DPE |
0.065 |
−0.113 |
0.243 |
≥0.25 |
ns |
5) Water temperature influences gut microbiome’s
sensitivity to parasite exposure
Fig. 5 Water temperature influences gut microbiome response to
Pseudocapillaria tomentosa. (A) Simpson’s Index for diversity of
parasite unexposed and pre-exposed fish at 0 days post exposure (dpe).
Prior to parasite exposure gut microbial alpha-diversity does not
significantly differ between fish reared at the same water temperature.
(B) Simpson’s Index for diversity of parasite unexposed and exposed
fish. Gut microbial alpha-diversity significantly differs between
parasite exposed fish reared at 28°C and 32°C water temperature relative
to unexposed control fish, but gut microbial alpha-diversity does not
differ between parasite unexposed and exposed fish reared at 35°C water
temperature. Capscale ordinations based on the Bray-Curtis dissimilarity
of gut microbiome composition constrained on the main and interaction
effects of temperature and parasite exposure (treatment) (C) of
pre-exposed samples at 0 dpe, and (D) post-exposed samples after 0 dpe.
The analysis shows that gut microbiome composition of fish significantly
differs between water temperature and parasite exposure in pre- and
post-exposed fish. However, the interaction effect between temperature
and parasite exposure is not significant in pre-exposed fish, but is
significant in post-exposed fish. Ribbons and ellipses indicate 95%
confidence interval. Ribbons and ellipses indicate 95% confidence
interval. Only statistically significant relationships are shown. A “*”
indicates statistical significance below the “0.05” level. Black arrows
indicate statistically significant covariates and direction of greatest
change in the indicated covariates.
5A)

Tables
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GLM
| GLM Results |
| glm(Alpha.Score ~ Temperature*Treatment); Pre-Exposed fish |
| term |
estimate |
std.error |
statistic |
p.value |
p.adj.sig |
| Shannon |
| (Intercept) |
−0.218 |
0.269 |
−0.811 |
≥0.25 |
ns |
| Temperature32 |
0.345 |
0.379 |
0.910 |
≥0.25 |
ns |
| Temperature35 |
0.693 |
0.384 |
1.803 |
0.077 |
ns |
| TreatmentExposed |
0.274 |
0.379 |
0.723 |
≥0.25 |
ns |
| Temperature32:TreatmentExposed |
0.311 |
0.544 |
0.572 |
≥0.25 |
ns |
| Temperature35:TreatmentExposed |
0.041 |
0.550 |
0.075 |
≥0.25 |
ns |
| Simpson |
| (Intercept) |
−0.241 |
0.252 |
−0.958 |
≥0.25 |
ns |
| Temperature32 |
0.352 |
0.355 |
0.990 |
≥0.25 |
ns |
| Temperature35 |
0.961 |
0.367 |
2.619 |
0.011 |
* |
| TreatmentExposed |
0.223 |
0.355 |
0.627 |
≥0.25 |
ns |
| Temperature32:TreatmentExposed |
0.486 |
0.513 |
0.948 |
≥0.25 |
ns |
| Temperature35:TreatmentExposed |
0.017 |
0.525 |
0.033 |
≥0.25 |
ns |
| Richness |
| (Intercept) |
−0.145 |
0.270 |
−0.538 |
≥0.25 |
ns |
| Temperature32 |
0.550 |
0.385 |
1.430 |
0.158 |
ns |
| Temperature35 |
0.043 |
0.381 |
0.112 |
≥0.25 |
ns |
| TreatmentExposed |
0.402 |
0.382 |
1.050 |
≥0.25 |
ns |
| Temperature32:TreatmentExposed |
0.020 |
0.554 |
0.036 |
≥0.25 |
ns |
| Temperature35:TreatmentExposed |
0.287 |
0.545 |
0.526 |
≥0.25 |
ns |
ANOVA
| ANOVA of GLM |
| ANOVA(GLM(Alpha.Score ~ Temperature*Treatment), type = 2); Pre-Exposed fish |
| term |
statistic |
df |
p.value |
sig |
| Shannon |
| Temperature |
7.264 |
2.000 |
0.026 |
* |
| Treatment |
3.044 |
1.000 |
0.081 |
ns |
| Temperature:Treatment |
0.380 |
2.000 |
≥0.25 |
ns |
| Simpson |
| Temperature |
14.462 |
2.000 |
<0.001 |
*** |
| Treatment |
3.406 |
1.000 |
0.065 |
ns |
| Temperature:Treatment |
1.122 |
2.000 |
≥0.25 |
ns |
| Richness |
| Temperature |
4.311 |
2.000 |
0.116 |
ns |
| Treatment |
5.067 |
1.000 |
0.024 |
* |
| Temperature:Treatment |
0.340 |
2.000 |
≥0.25 |
ns |
Tukey
| Pairwise Tukey's HSD, p.adj: Dunnett |
| Tukey(Alpha.Score ~ Temperature*Treatment); Pre-Exposed fish |
| Temperature |
.y. |
term |
group1 |
group2 |
estimate |
std.error |
statistic |
adj.p.value |
Variable |
Group |
| Shannon |
| 28 |
Alpha.Score |
Treatment |
Exposed |
Control |
0.274 |
0.364 |
0.753 |
≥0.25 |
Treatment |
28 |
| 32 |
Alpha.Score |
Treatment |
Exposed |
Control |
0.585 |
0.451 |
1.298 |
0.194 |
Treatment |
32 |
| 35 |
Alpha.Score |
Treatment |
Exposed |
Control |
0.315 |
0.344 |
0.917 |
≥0.25 |
Treatment |
35 |
| Simpson |
| 28 |
Alpha.Score |
Treatment |
Exposed |
Control |
0.223 |
0.337 |
0.662 |
≥0.25 |
Treatment |
28 |
| 32 |
Alpha.Score |
Treatment |
Exposed |
Control |
0.709 |
0.426 |
1.663 |
0.096 |
Treatment |
32 |
| 35 |
Alpha.Score |
Treatment |
Exposed |
Control |
0.240 |
0.339 |
0.708 |
≥0.25 |
Treatment |
35 |
| Richness |
| 28 |
Alpha.Score |
Treatment |
Exposed |
Control |
0.402 |
0.361 |
1.112 |
≥0.25 |
Treatment |
28 |
| 32 |
Alpha.Score |
Treatment |
Exposed |
Control |
0.422 |
0.411 |
1.027 |
≥0.25 |
Treatment |
32 |
| 35 |
Alpha.Score |
Treatment |
Exposed |
Control |
0.689 |
0.400 |
1.721 |
0.085 |
Treatment |
35 |
5B)

Tables
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GLM
| GLM Results |
| glm(Alpha.Score ~ Temperature*Treatment); Post-Exposed fish |
| term |
estimate |
std.error |
statistic |
p.value |
p.adj.sig |
| Shannon |
| (Intercept) |
−0.441 |
0.126 |
−3.499 |
<0.001 |
*** |
| Temperature32 |
−0.064 |
0.177 |
−0.364 |
≥0.25 |
ns |
| Temperature35 |
−0.087 |
0.182 |
−0.476 |
≥0.25 |
ns |
| TreatmentExposed |
−0.539 |
0.210 |
−2.562 |
0.011 |
* |
| Temperature32:TreatmentExposed |
0.953 |
0.280 |
3.405 |
<0.001 |
*** |
| Temperature35:TreatmentExposed |
0.561 |
0.281 |
1.996 |
0.047 |
* |
| Simpson |
| (Intercept) |
−0.299 |
0.133 |
−2.252 |
0.025 |
* |
| Temperature32 |
−0.059 |
0.186 |
−0.319 |
≥0.25 |
ns |
| Temperature35 |
0.080 |
0.190 |
0.419 |
≥0.25 |
ns |
| TreatmentExposed |
−0.724 |
0.225 |
−3.223 |
0.001 |
** |
| Temperature32:TreatmentExposed |
1.106 |
0.298 |
3.715 |
<0.001 |
*** |
| Temperature35:TreatmentExposed |
0.673 |
0.297 |
2.270 |
0.024 |
* |
| Richness |
| (Intercept) |
−0.206 |
0.101 |
−2.040 |
0.043 |
* |
| Temperature32 |
−0.048 |
0.141 |
−0.343 |
≥0.25 |
ns |
| Temperature35 |
−0.317 |
0.147 |
−2.164 |
0.032 |
* |
| TreatmentExposed |
−0.228 |
0.160 |
−1.421 |
0.157 |
ns |
| Temperature32:TreatmentExposed |
0.829 |
0.219 |
3.776 |
<0.001 |
*** |
| Temperature35:TreatmentExposed |
0.691 |
0.219 |
3.155 |
0.002 |
** |
ANOVA
| ANOVA of GLM |
| ANOVA(GLM(Alpha.Score ~ Temperature*Treatment), type = 2); Post-Exposed fish |
| term |
statistic |
df |
p.value |
sig |
| Shannon |
| Temperature |
5.908 |
2.000 |
0.052 |
ns |
| Treatment |
0.001 |
1.000 |
≥0.25 |
ns |
| Temperature:Treatment |
11.784 |
2.000 |
0.003 |
** |
| Simpson |
| Temperature |
8.400 |
2.000 |
0.015 |
* |
| Treatment |
0.547 |
1.000 |
≥0.25 |
ns |
| Temperature:Treatment |
14.128 |
2.000 |
<0.001 |
*** |
| Richness |
| Temperature |
11.325 |
2.000 |
0.003 |
** |
| Treatment |
11.824 |
1.000 |
<0.001 |
*** |
| Temperature:Treatment |
16.178 |
2.000 |
<0.001 |
*** |
Tukey
| Pairwise Tukey's HSD, p.adj: Dunnett |
| Tukey(Alpha.Score ~ Temperature*Treatment); Post-Exposed fish |
| Temperature |
.y. |
term |
group1 |
group2 |
estimate |
std.error |
statistic |
adj.p.value |
Variable |
Group |
| Shannon |
| 28 |
Alpha.Score |
Treatment |
Exposed |
Control |
−0.539 |
0.280 |
−1.924 |
0.054 |
Treatment |
28 |
| 32 |
Alpha.Score |
Treatment |
Exposed |
Control |
0.414 |
0.180 |
2.298 |
0.022 |
Treatment |
32 |
| 35 |
Alpha.Score |
Treatment |
Exposed |
Control |
0.022 |
0.111 |
0.200 |
≥0.25 |
Treatment |
35 |
| Simpson |
| 28 |
Alpha.Score |
Treatment |
Exposed |
Control |
−0.724 |
0.290 |
−2.492 |
0.013 |
Treatment |
28 |
| 32 |
Alpha.Score |
Treatment |
Exposed |
Control |
0.382 |
0.190 |
2.013 |
0.044 |
Treatment |
32 |
| 35 |
Alpha.Score |
Treatment |
Exposed |
Control |
−0.051 |
0.130 |
−0.387 |
≥0.25 |
Treatment |
35 |
| Richness |
| 28 |
Alpha.Score |
Treatment |
Exposed |
Control |
−0.228 |
0.223 |
−1.021 |
≥0.25 |
Treatment |
28 |
| 32 |
Alpha.Score |
Treatment |
Exposed |
Control |
0.601 |
0.132 |
4.546 |
<0.001 |
Treatment |
32 |
| 35 |
Alpha.Score |
Treatment |
Exposed |
Control |
0.463 |
0.092 |
5.021 |
<0.001 |
Treatment |
35 |
5C)

Tables
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Capscale
| ADONIS2 |
| adonis2(Beta Distance ~ Temperature*Treatment); Pre-Exposed fish |
| term |
df |
SumOfSqs |
R2 |
statistic |
p.value |
sig |
| bray |
| Temperature |
2.000 |
0.871 |
0.188 |
7.178 |
0.001 |
*** |
| Treatment |
1.000 |
0.244 |
0.053 |
4.025 |
0.010 |
** |
| Temperature:Treatment |
2.000 |
0.245 |
0.053 |
2.019 |
0.053 |
ns |
| Residual |
54.000 |
3.275 |
0.707 |
NA |
NA |
NA |
| Total |
59.000 |
4.635 |
1.000 |
NA |
NA |
NA |
| canberra |
| Temperature |
2.000 |
1.652 |
0.132 |
4.615 |
0.001 |
*** |
| Treatment |
1.000 |
0.533 |
0.043 |
2.979 |
0.001 |
*** |
| Temperature:Treatment |
2.000 |
0.667 |
0.053 |
1.864 |
0.001 |
*** |
| Residual |
54.000 |
9.665 |
0.772 |
NA |
NA |
NA |
| Total |
59.000 |
12.517 |
1.000 |
NA |
NA |
NA |
| gunifrac |
| Temperature |
2.000 |
0.647 |
0.153 |
5.604 |
0.001 |
*** |
| Treatment |
1.000 |
0.288 |
0.068 |
4.987 |
0.003 |
** |
| Temperature:Treatment |
2.000 |
0.177 |
0.042 |
1.537 |
0.109 |
ns |
| Residual |
54.000 |
3.117 |
0.737 |
NA |
NA |
NA |
| Total |
59.000 |
4.229 |
1.000 |
NA |
NA |
NA |
Dispersion (ANOVA)
| ANOVA: Homogeneity of Dispersion |
| ANOVA(Beta Disperson ~ Temperature); Pre-Exposed fish |
| term |
df |
sumsq |
meansq |
statistic |
p.value |
sig |
| bray |
| Temp.Treat |
5.000 |
0.103 |
0.021 |
2.738 |
0.028 |
* |
| Residual |
54.000 |
0.405 |
0.007 |
NA |
NA |
NA |
| canberra |
| Temp.Treat |
5.000 |
0.014 |
0.003 |
0.453 |
≥0.25 |
ns |
| Residual |
54.000 |
0.329 |
0.006 |
NA |
NA |
NA |
| gunifrac |
| Temp.Treat |
5.000 |
0.054 |
0.011 |
1.564 |
0.186 |
ns |
| Residual |
54.000 |
0.369 |
0.007 |
NA |
NA |
NA |
Dispersion (Tukey)
| Tukey: Homogeneity of Dispersion |
| Tukey(Beta Disperson ~ Temperature*Treatment); Pre-Exposed fish |
| .y. |
term |
group1 |
group2 |
estimate |
conf.low |
conf.high |
adj.p.value |
sig |
| bray |
| Distance |
Temp.Treat |
28°C__Exposed |
28°C__Control |
0.027 |
−0.088 |
0.141 |
≥0.25 |
ns |
| Distance |
Temp.Treat |
32°C__Control |
28°C__Control |
0.095 |
−0.019 |
0.210 |
0.153 |
ns |
| Distance |
Temp.Treat |
32°C__Exposed |
28°C__Control |
0.110 |
−0.004 |
0.225 |
0.065 |
ns |
| Distance |
Temp.Treat |
35°C__Control |
28°C__Control |
0.015 |
−0.099 |
0.130 |
≥0.25 |
ns |
| Distance |
Temp.Treat |
35°C__Exposed |
28°C__Control |
0.069 |
−0.045 |
0.183 |
≥0.25 |
ns |
| Distance |
Temp.Treat |
32°C__Control |
28°C__Exposed |
0.069 |
−0.046 |
0.183 |
≥0.25 |
ns |
| Distance |
Temp.Treat |
32°C__Exposed |
28°C__Exposed |
0.084 |
−0.031 |
0.198 |
≥0.25 |
ns |
| Distance |
Temp.Treat |
35°C__Control |
28°C__Exposed |
−0.011 |
−0.126 |
0.103 |
≥0.25 |
ns |
| Distance |
Temp.Treat |
35°C__Exposed |
28°C__Exposed |
0.042 |
−0.072 |
0.157 |
≥0.25 |
ns |
| Distance |
Temp.Treat |
32°C__Exposed |
32°C__Control |
0.015 |
−0.100 |
0.129 |
≥0.25 |
ns |
| Distance |
Temp.Treat |
35°C__Control |
32°C__Control |
−0.080 |
−0.195 |
0.034 |
≥0.25 |
ns |
| Distance |
Temp.Treat |
35°C__Exposed |
32°C__Control |
−0.026 |
−0.141 |
0.088 |
≥0.25 |
ns |
| Distance |
Temp.Treat |
35°C__Control |
32°C__Exposed |
−0.095 |
−0.209 |
0.019 |
0.156 |
ns |
| Distance |
Temp.Treat |
35°C__Exposed |
32°C__Exposed |
−0.041 |
−0.156 |
0.073 |
≥0.25 |
ns |
| Distance |
Temp.Treat |
35°C__Exposed |
35°C__Control |
0.054 |
−0.061 |
0.168 |
≥0.25 |
ns |
| canberra |
| Distance |
Temp.Treat |
28°C__Exposed |
28°C__Control |
0.011 |
−0.092 |
0.114 |
≥0.25 |
ns |
| Distance |
Temp.Treat |
32°C__Control |
28°C__Control |
0.035 |
−0.068 |
0.138 |
≥0.25 |
ns |
| Distance |
Temp.Treat |
32°C__Exposed |
28°C__Control |
0.044 |
−0.059 |
0.147 |
≥0.25 |
ns |
| Distance |
Temp.Treat |
35°C__Control |
28°C__Control |
0.010 |
−0.094 |
0.113 |
≥0.25 |
ns |
| Distance |
Temp.Treat |
35°C__Exposed |
28°C__Control |
0.016 |
−0.087 |
0.119 |
≥0.25 |
ns |
| Distance |
Temp.Treat |
32°C__Control |
28°C__Exposed |
0.024 |
−0.079 |
0.127 |
≥0.25 |
ns |
| Distance |
Temp.Treat |
32°C__Exposed |
28°C__Exposed |
0.033 |
−0.070 |
0.136 |
≥0.25 |
ns |
| Distance |
Temp.Treat |
35°C__Control |
28°C__Exposed |
−0.001 |
−0.104 |
0.102 |
≥0.25 |
ns |
| Distance |
Temp.Treat |
35°C__Exposed |
28°C__Exposed |
0.005 |
−0.098 |
0.108 |
≥0.25 |
ns |
| Distance |
Temp.Treat |
32°C__Exposed |
32°C__Control |
0.009 |
−0.094 |
0.112 |
≥0.25 |
ns |
| Distance |
Temp.Treat |
35°C__Control |
32°C__Control |
−0.025 |
−0.128 |
0.078 |
≥0.25 |
ns |
| Distance |
Temp.Treat |
35°C__Exposed |
32°C__Control |
−0.019 |
−0.122 |
0.084 |
≥0.25 |
ns |
| Distance |
Temp.Treat |
35°C__Control |
32°C__Exposed |
−0.034 |
−0.137 |
0.069 |
≥0.25 |
ns |
| Distance |
Temp.Treat |
35°C__Exposed |
32°C__Exposed |
−0.028 |
−0.131 |
0.075 |
≥0.25 |
ns |
| Distance |
Temp.Treat |
35°C__Exposed |
35°C__Control |
0.006 |
−0.097 |
0.109 |
≥0.25 |
ns |
| gunifrac |
| Distance |
Temp.Treat |
28°C__Exposed |
28°C__Control |
0.003 |
−0.106 |
0.112 |
≥0.25 |
ns |
| Distance |
Temp.Treat |
32°C__Control |
28°C__Control |
0.051 |
−0.058 |
0.160 |
≥0.25 |
ns |
| Distance |
Temp.Treat |
32°C__Exposed |
28°C__Control |
0.078 |
−0.032 |
0.187 |
≥0.25 |
ns |
| Distance |
Temp.Treat |
35°C__Control |
28°C__Control |
0.000 |
−0.109 |
0.110 |
≥0.25 |
ns |
| Distance |
Temp.Treat |
35°C__Exposed |
28°C__Control |
0.042 |
−0.067 |
0.151 |
≥0.25 |
ns |
| Distance |
Temp.Treat |
32°C__Control |
28°C__Exposed |
0.048 |
−0.061 |
0.158 |
≥0.25 |
ns |
| Distance |
Temp.Treat |
32°C__Exposed |
28°C__Exposed |
0.075 |
−0.035 |
0.184 |
≥0.25 |
ns |
| Distance |
Temp.Treat |
35°C__Control |
28°C__Exposed |
−0.002 |
−0.112 |
0.107 |
≥0.25 |
ns |
| Distance |
Temp.Treat |
35°C__Exposed |
28°C__Exposed |
0.039 |
−0.070 |
0.148 |
≥0.25 |
ns |
| Distance |
Temp.Treat |
32°C__Exposed |
32°C__Control |
0.026 |
−0.083 |
0.136 |
≥0.25 |
ns |
| Distance |
Temp.Treat |
35°C__Control |
32°C__Control |
−0.051 |
−0.160 |
0.059 |
≥0.25 |
ns |
| Distance |
Temp.Treat |
35°C__Exposed |
32°C__Control |
−0.009 |
−0.118 |
0.100 |
≥0.25 |
ns |
| Distance |
Temp.Treat |
35°C__Control |
32°C__Exposed |
−0.077 |
−0.186 |
0.032 |
≥0.25 |
ns |
| Distance |
Temp.Treat |
35°C__Exposed |
32°C__Exposed |
−0.036 |
−0.145 |
0.074 |
≥0.25 |
ns |
| Distance |
Temp.Treat |
35°C__Exposed |
35°C__Control |
0.041 |
−0.068 |
0.151 |
≥0.25 |
ns |
5D)

Tables
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Capscale
| ADONIS2 |
| adonis2(Beta Distance ~ Temperature*Treatment); Post-Exposed fish |
| term |
df |
SumOfSqs |
R2 |
statistic |
p.value |
sig |
| bray |
| Temperature |
2.000 |
4.123 |
0.108 |
17.035 |
0.001 |
*** |
| Treatment |
1.000 |
7.411 |
0.194 |
61.235 |
0.001 |
*** |
| Temperature:Treatment |
2.000 |
3.168 |
0.083 |
13.088 |
0.001 |
*** |
| Residual |
194.000 |
23.478 |
0.615 |
NA |
NA |
NA |
| Total |
199.000 |
38.179 |
1.000 |
NA |
NA |
NA |
| canberra |
| Temperature |
2.000 |
4.561 |
0.074 |
9.502 |
0.001 |
*** |
| Treatment |
1.000 |
7.402 |
0.120 |
30.840 |
0.001 |
*** |
| Temperature:Treatment |
2.000 |
3.014 |
0.049 |
6.279 |
0.001 |
*** |
| Residual |
194.000 |
46.561 |
0.757 |
NA |
NA |
NA |
| Total |
199.000 |
61.538 |
1.000 |
NA |
NA |
NA |
| gunifrac |
| Temperature |
2.000 |
2.273 |
0.082 |
11.945 |
0.001 |
*** |
| Treatment |
1.000 |
4.718 |
0.170 |
49.577 |
0.001 |
*** |
| Temperature:Treatment |
2.000 |
2.273 |
0.082 |
11.944 |
0.001 |
*** |
| Residual |
194.000 |
18.460 |
0.666 |
NA |
NA |
NA |
| Total |
199.000 |
27.724 |
1.000 |
NA |
NA |
NA |
Dispersion (ANOVA)
| ANOVA: Homogeneity of Dispersion |
| ANOVA(Beta Disperson ~ Temperature); Post-Exposed fish |
| term |
df |
sumsq |
meansq |
statistic |
p.value |
sig |
| bray |
| Temp.Treat |
5.000 |
1.344 |
0.269 |
17.882 |
<0.001 |
**** |
| Residual |
194.000 |
2.917 |
0.015 |
NA |
NA |
NA |
| canberra |
| Temp.Treat |
5.000 |
1.094 |
0.219 |
31.758 |
<0.001 |
**** |
| Residual |
194.000 |
1.337 |
0.007 |
NA |
NA |
NA |
| gunifrac |
| Temp.Treat |
5.000 |
1.225 |
0.245 |
29.264 |
<0.001 |
**** |
| Residual |
194.000 |
1.624 |
0.008 |
NA |
NA |
NA |
Dispersion (Tukey)
| Tukey: Homogeneity of Dispersion |
| Tukey(Beta Disperson ~ Temperature*Treatment); Post-Exposed fish |
| .y. |
term |
group1 |
group2 |
estimate |
conf.low |
conf.high |
adj.p.value |
sig |
| bray |
| Distance |
Temp.Treat |
28°C__Exposed |
28°C__Control |
0.211 |
0.119 |
0.302 |
<0.001 |
**** |
| Distance |
Temp.Treat |
32°C__Control |
28°C__Control |
−0.015 |
−0.096 |
0.066 |
≥0.25 |
ns |
| Distance |
Temp.Treat |
32°C__Exposed |
28°C__Control |
0.168 |
0.081 |
0.255 |
<0.001 |
**** |
| Distance |
Temp.Treat |
35°C__Control |
28°C__Control |
0.013 |
−0.070 |
0.096 |
≥0.25 |
ns |
| Distance |
Temp.Treat |
35°C__Exposed |
28°C__Control |
0.060 |
−0.024 |
0.144 |
≥0.25 |
ns |
| Distance |
Temp.Treat |
32°C__Control |
28°C__Exposed |
−0.225 |
−0.316 |
−0.135 |
<0.001 |
**** |
| Distance |
Temp.Treat |
32°C__Exposed |
28°C__Exposed |
−0.043 |
−0.138 |
0.053 |
≥0.25 |
ns |
| Distance |
Temp.Treat |
35°C__Control |
28°C__Exposed |
−0.197 |
−0.290 |
−0.105 |
<0.001 |
**** |
| Distance |
Temp.Treat |
35°C__Exposed |
28°C__Exposed |
−0.150 |
−0.243 |
−0.057 |
<0.001 |
**** |
| Distance |
Temp.Treat |
32°C__Exposed |
32°C__Control |
0.183 |
0.097 |
0.268 |
<0.001 |
**** |
| Distance |
Temp.Treat |
35°C__Control |
32°C__Control |
0.028 |
−0.054 |
0.110 |
≥0.25 |
ns |
| Distance |
Temp.Treat |
35°C__Exposed |
32°C__Control |
0.075 |
−0.008 |
0.158 |
0.100 |
ns |
| Distance |
Temp.Treat |
35°C__Control |
32°C__Exposed |
−0.155 |
−0.243 |
−0.067 |
<0.001 |
**** |
| Distance |
Temp.Treat |
35°C__Exposed |
32°C__Exposed |
−0.108 |
−0.196 |
−0.019 |
0.007 |
** |
| Distance |
Temp.Treat |
35°C__Exposed |
35°C__Control |
0.047 |
−0.038 |
0.132 |
≥0.25 |
ns |
| canberra |
| Distance |
Temp.Treat |
28°C__Exposed |
28°C__Control |
0.217 |
0.155 |
0.279 |
<0.001 |
**** |
| Distance |
Temp.Treat |
32°C__Control |
28°C__Control |
−0.011 |
−0.066 |
0.044 |
≥0.25 |
ns |
| Distance |
Temp.Treat |
32°C__Exposed |
28°C__Control |
0.107 |
0.048 |
0.166 |
<0.001 |
**** |
| Distance |
Temp.Treat |
35°C__Control |
28°C__Control |
0.013 |
−0.044 |
0.069 |
≥0.25 |
ns |
| Distance |
Temp.Treat |
35°C__Exposed |
28°C__Control |
0.073 |
0.016 |
0.130 |
0.004 |
** |
| Distance |
Temp.Treat |
32°C__Control |
28°C__Exposed |
−0.228 |
−0.289 |
−0.167 |
<0.001 |
**** |
| Distance |
Temp.Treat |
32°C__Exposed |
28°C__Exposed |
−0.110 |
−0.175 |
−0.045 |
<0.001 |
**** |
| Distance |
Temp.Treat |
35°C__Control |
28°C__Exposed |
−0.204 |
−0.267 |
−0.142 |
<0.001 |
**** |
| Distance |
Temp.Treat |
35°C__Exposed |
28°C__Exposed |
−0.144 |
−0.207 |
−0.081 |
<0.001 |
**** |
| Distance |
Temp.Treat |
32°C__Exposed |
32°C__Control |
0.118 |
0.060 |
0.176 |
<0.001 |
**** |
| Distance |
Temp.Treat |
35°C__Control |
32°C__Control |
0.024 |
−0.032 |
0.080 |
≥0.25 |
ns |
| Distance |
Temp.Treat |
35°C__Exposed |
32°C__Control |
0.084 |
0.028 |
0.140 |
<0.001 |
*** |
| Distance |
Temp.Treat |
35°C__Control |
32°C__Exposed |
−0.094 |
−0.154 |
−0.035 |
<0.001 |
*** |
| Distance |
Temp.Treat |
35°C__Exposed |
32°C__Exposed |
−0.034 |
−0.094 |
0.026 |
≥0.25 |
ns |
| Distance |
Temp.Treat |
35°C__Exposed |
35°C__Control |
0.060 |
0.003 |
0.118 |
0.035 |
* |
| gunifrac |
| Distance |
Temp.Treat |
28°C__Exposed |
28°C__Control |
0.197 |
0.129 |
0.266 |
<0.001 |
**** |
| Distance |
Temp.Treat |
32°C__Control |
28°C__Control |
−0.023 |
−0.083 |
0.037 |
≥0.25 |
ns |
| Distance |
Temp.Treat |
32°C__Exposed |
28°C__Control |
0.141 |
0.077 |
0.206 |
<0.001 |
**** |
| Distance |
Temp.Treat |
35°C__Control |
28°C__Control |
−0.011 |
−0.073 |
0.051 |
≥0.25 |
ns |
| Distance |
Temp.Treat |
35°C__Exposed |
28°C__Control |
0.054 |
−0.009 |
0.117 |
0.134 |
ns |
| Distance |
Temp.Treat |
32°C__Control |
28°C__Exposed |
−0.220 |
−0.288 |
−0.153 |
<0.001 |
**** |
| Distance |
Temp.Treat |
32°C__Exposed |
28°C__Exposed |
−0.056 |
−0.128 |
0.015 |
0.211 |
ns |
| Distance |
Temp.Treat |
35°C__Control |
28°C__Exposed |
−0.209 |
−0.278 |
−0.140 |
<0.001 |
**** |
| Distance |
Temp.Treat |
35°C__Exposed |
28°C__Exposed |
−0.143 |
−0.213 |
−0.074 |
<0.001 |
**** |
| Distance |
Temp.Treat |
32°C__Exposed |
32°C__Control |
0.164 |
0.100 |
0.228 |
<0.001 |
**** |
| Distance |
Temp.Treat |
35°C__Control |
32°C__Control |
0.012 |
−0.050 |
0.073 |
≥0.25 |
ns |
| Distance |
Temp.Treat |
35°C__Exposed |
32°C__Control |
0.077 |
0.015 |
0.139 |
0.006 |
** |
| Distance |
Temp.Treat |
35°C__Control |
32°C__Exposed |
−0.152 |
−0.218 |
−0.087 |
<0.001 |
**** |
| Distance |
Temp.Treat |
35°C__Exposed |
32°C__Exposed |
−0.087 |
−0.153 |
−0.021 |
0.003 |
** |
| Distance |
Temp.Treat |
35°C__Exposed |
35°C__Control |
0.065 |
0.002 |
0.129 |
0.039 |
* |
6) Water temperature moderates the impact of
infection burden on fish gut microbiomes
Fig. 6 Exposure to Pseudocapillaria tomentosa differentially impacts
gut microbiome depending on water temperature. (A) Simpson’s Index for
diversity of parasite exposed fish. Gut microbial alpha-diversity does
not significantly differ between fish reared at the same water
temperature with presence or absence of infection. (B) Capscale
ordination based on the Canberra dissimilarity of gut microbiome
composition of parasite exposed fish. The analysis shows that gut
microbiome composition significantly differs between positively infected
fish reared at different water temperatures. (C) Total worm count is
positively correlated with lowest or highest alpha diversity scores in
fish with detectable parasites. Points are colored by temperature and
filled by cluster (High = purple, Low = orange, Other = white), and
samples with a worm count equal to 0 are transparent. (D) Capscale
ordination based on the Bray-Curtis dissimilarity of gut microbiome
composition of water temperature and worm counts. Points are colored by
temperature and filled by cluster (High = purple, Low = orange, Other =
white), and samples with a worm count equal to 0 are transparent. The
analysis shows that gut microbiome composition significantly differs
between water temperature and total worm count, and samples labeled as
“High” or “Low” form distinct clusters. Ribbons and ellipses indicate
95% confidence interval. Only statistically significant relationships
are shown. A “*” indicates statistical significance below the “0.05”
level. Black arrows indicate statistically significant covariates and
direction of greatest change in the indicated covariates.
6A)

Tables
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GLM
| GLM Results |
| glm(Alpha.Score ~ Temperature*Pathology.Results); Exposed fish |
| term |
estimate |
std.error |
statistic |
p.value |
p.adj.sig |
| Shannon |
| (Intercept) |
−1.090 |
0.429 |
−2.544 |
0.013 |
* |
| Temperature32 |
0.889 |
0.508 |
1.749 |
0.084 |
ns |
| Temperature35 |
0.693 |
0.468 |
1.482 |
0.142 |
ns |
| Pathology.Resultspositive |
0.223 |
0.512 |
0.436 |
≥0.25 |
ns |
| Temperature32:Pathology.Resultspositive |
0.125 |
0.641 |
0.195 |
≥0.25 |
ns |
| Temperature35:Pathology.Resultspositive |
−1.122 |
1.392 |
−0.806 |
≥0.25 |
ns |
| Simpson |
| (Intercept) |
−1.265 |
0.458 |
−2.760 |
0.007 |
** |
| Temperature32 |
1.105 |
0.536 |
2.061 |
0.042 |
* |
| Temperature35 |
1.055 |
0.495 |
2.130 |
0.036 |
* |
| Pathology.Resultspositive |
0.375 |
0.541 |
0.694 |
≥0.25 |
ns |
| Temperature32:Pathology.Resultspositive |
0.054 |
0.669 |
0.081 |
≥0.25 |
ns |
| Temperature35:Pathology.Resultspositive |
−1.154 |
1.337 |
−0.863 |
≥0.25 |
ns |
| Richness |
| (Intercept) |
−0.421 |
0.329 |
−1.279 |
0.204 |
ns |
| Temperature32 |
0.483 |
0.405 |
1.194 |
0.236 |
ns |
| Temperature35 |
−0.002 |
0.367 |
−0.005 |
≥0.25 |
ns |
| Pathology.Resultspositive |
−0.390 |
0.407 |
−0.957 |
≥0.25 |
ns |
| Temperature32:Pathology.Resultspositive |
0.310 |
0.526 |
0.589 |
≥0.25 |
ns |
| Temperature35:Pathology.Resultspositive |
−0.221 |
1.125 |
−0.197 |
≥0.25 |
ns |
ANOVA
| ANOVA of GLM |
| ANOVA(GLM(Alpha.Score ~ Temperature*Time), type = 2); Exposed fish |
| term |
statistic |
df |
p.value |
sig |
| Shannon |
| Temperature |
9.895 |
2.000 |
0.007 |
** |
| Pathology.Results |
0.569 |
1.000 |
≥0.25 |
ns |
| Temperature:Pathology.Results |
0.997 |
2.000 |
≥0.25 |
ns |
| Simpson |
| Temperature |
13.663 |
2.000 |
0.001 |
** |
| Pathology.Results |
1.134 |
1.000 |
≥0.25 |
ns |
| Temperature:Pathology.Results |
0.999 |
2.000 |
≥0.25 |
ns |
| Richness |
| Temperature |
8.625 |
2.000 |
0.013 |
* |
| Pathology.Results |
0.840 |
1.000 |
≥0.25 |
ns |
| Temperature:Pathology.Results |
0.495 |
2.000 |
≥0.25 |
ns |
Tukey
| Pairwise Tukey's HSD, p.adj: Dunnett |
| Tukey(Alpha.Score ~ Temperature*Time); Exposed fish |
| Temperature |
.y. |
term |
group1 |
group2 |
estimate |
std.error |
statistic |
adj.p.value |
Variable |
Group |
| Shannon |
| 28 |
Alpha.Score |
Pathology.Results |
positive |
negative |
0.223 |
0.732 |
0.305 |
≥0.25 |
Pathology.Results |
28 |
| 32 |
Alpha.Score |
Pathology.Results |
positive |
negative |
0.348 |
0.383 |
0.908 |
≥0.25 |
Pathology.Results |
32 |
| 35 |
Alpha.Score |
Pathology.Results |
positive |
negative |
−0.899 |
0.659 |
−1.365 |
0.172 |
Pathology.Results |
35 |
| Simpson |
| 28 |
Alpha.Score |
Pathology.Results |
positive |
negative |
0.375 |
0.754 |
0.497 |
≥0.25 |
Pathology.Results |
28 |
| 32 |
Alpha.Score |
Pathology.Results |
positive |
negative |
0.429 |
0.394 |
1.089 |
≥0.25 |
Pathology.Results |
32 |
| 35 |
Alpha.Score |
Pathology.Results |
positive |
negative |
−0.779 |
0.692 |
−1.125 |
≥0.25 |
Pathology.Results |
35 |
| Richness |
| 28 |
Alpha.Score |
Pathology.Results |
positive |
negative |
−0.390 |
0.608 |
−0.641 |
≥0.25 |
Pathology.Results |
28 |
| 32 |
Alpha.Score |
Pathology.Results |
positive |
negative |
−0.080 |
0.301 |
−0.267 |
≥0.25 |
Pathology.Results |
32 |
| 35 |
Alpha.Score |
Pathology.Results |
positive |
negative |
−0.611 |
0.552 |
−1.107 |
≥0.25 |
Pathology.Results |
35 |
6B)

Tables
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Capscale
| ADONIS2 |
| adonis2(Beta Distance ~ Temperature*Pathology.Results); Exposed fish |
| term |
df |
SumOfSqs |
R2 |
statistic |
p.value |
sig |
| bray |
| Temperature |
2.000 |
5.218 |
0.251 |
14.569 |
0.001 |
*** |
| Pathology.Results |
1.000 |
0.263 |
0.013 |
1.471 |
0.185 |
ns |
| Temperature:Pathology.Results |
2.000 |
0.404 |
0.019 |
1.127 |
≥0.25 |
ns |
| Residual |
83.000 |
14.863 |
0.716 |
NA |
NA |
NA |
| Total |
88.000 |
20.748 |
1.000 |
NA |
NA |
NA |
| canberra |
| Temperature |
2.000 |
3.573 |
0.117 |
5.751 |
0.001 |
*** |
| Pathology.Results |
1.000 |
0.488 |
0.016 |
1.570 |
0.031 |
* |
| Temperature:Pathology.Results |
2.000 |
0.679 |
0.022 |
1.093 |
≥0.25 |
ns |
| Residual |
83.000 |
25.781 |
0.845 |
NA |
NA |
NA |
| Total |
88.000 |
30.520 |
1.000 |
NA |
NA |
NA |
| gunifrac |
| Temperature |
2.000 |
3.363 |
0.212 |
11.651 |
0.001 |
*** |
| Pathology.Results |
1.000 |
0.234 |
0.015 |
1.622 |
0.149 |
ns |
| Temperature:Pathology.Results |
2.000 |
0.264 |
0.017 |
0.915 |
≥0.25 |
ns |
| Residual |
83.000 |
11.980 |
0.756 |
NA |
NA |
NA |
| Total |
88.000 |
15.841 |
1.000 |
NA |
NA |
NA |
Dispersion (ANOVA)
| ANOVA: Homogeneity of Dispersion |
| ANOVA(Beta Disperson ~ Temperature*Pathology.Results); Exposed fish |
| term |
df |
sumsq |
meansq |
statistic |
p.value |
sig |
| bray |
| Temp.Path |
5.000 |
0.520 |
0.104 |
4.652 |
<0.001 |
*** |
| Residual |
83.000 |
1.855 |
0.022 |
NA |
NA |
NA |
| canberra |
| Temp.Path |
5.000 |
0.625 |
0.125 |
13.830 |
<0.001 |
**** |
| Residual |
83.000 |
0.750 |
0.009 |
NA |
NA |
NA |
| gunifrac |
| Temp.Path |
5.000 |
0.436 |
0.087 |
6.954 |
<0.001 |
**** |
| Residual |
83.000 |
1.040 |
0.013 |
NA |
NA |
NA |
Dispersion (Tukey)
| Tukey: Homogeneity of Dispersion |
| Tukey(Beta Disperson ~ Temperature*Pathology.Results); Exposed fish |
| .y. |
term |
group1 |
group2 |
estimate |
conf.low |
conf.high |
adj.p.value |
sig |
| bray |
| Distance |
Temp.Path |
28°C__pos |
28°C__neg |
0.022 |
−0.165 |
0.209 |
≥0.25 |
ns |
| Distance |
Temp.Path |
32°C__neg |
28°C__neg |
−0.086 |
−0.277 |
0.105 |
≥0.25 |
ns |
| Distance |
Temp.Path |
32°C__pos |
28°C__neg |
0.015 |
−0.176 |
0.206 |
≥0.25 |
ns |
| Distance |
Temp.Path |
35°C__neg |
28°C__neg |
−0.126 |
−0.298 |
0.046 |
≥0.25 |
ns |
| Distance |
Temp.Path |
35°C__pos |
28°C__neg |
−0.434 |
−0.896 |
0.029 |
0.079 |
ns |
| Distance |
Temp.Path |
32°C__neg |
28°C__pos |
−0.108 |
−0.263 |
0.046 |
≥0.25 |
ns |
| Distance |
Temp.Path |
32°C__pos |
28°C__pos |
−0.007 |
−0.161 |
0.148 |
≥0.25 |
ns |
| Distance |
Temp.Path |
35°C__neg |
28°C__pos |
−0.148 |
−0.278 |
−0.017 |
0.017 |
* |
| Distance |
Temp.Path |
35°C__pos |
28°C__pos |
−0.456 |
−0.905 |
−0.007 |
0.044 |
* |
| Distance |
Temp.Path |
32°C__pos |
32°C__neg |
0.101 |
−0.058 |
0.261 |
≥0.25 |
ns |
| Distance |
Temp.Path |
35°C__neg |
32°C__neg |
−0.039 |
−0.175 |
0.096 |
≥0.25 |
ns |
| Distance |
Temp.Path |
35°C__pos |
32°C__neg |
−0.348 |
−0.798 |
0.103 |
0.226 |
ns |
| Distance |
Temp.Path |
35°C__neg |
32°C__pos |
−0.141 |
−0.277 |
−0.005 |
0.038 |
* |
| Distance |
Temp.Path |
35°C__pos |
32°C__pos |
−0.449 |
−0.899 |
0.001 |
0.051 |
ns |
| Distance |
Temp.Path |
35°C__pos |
35°C__neg |
−0.308 |
−0.751 |
0.135 |
≥0.25 |
ns |
| canberra |
| Distance |
Temp.Path |
28°C__pos |
28°C__neg |
0.045 |
−0.074 |
0.164 |
≥0.25 |
ns |
| Distance |
Temp.Path |
32°C__neg |
28°C__neg |
−0.123 |
−0.244 |
−0.002 |
0.045 |
* |
| Distance |
Temp.Path |
32°C__pos |
28°C__neg |
−0.042 |
−0.164 |
0.079 |
≥0.25 |
ns |
| Distance |
Temp.Path |
35°C__neg |
28°C__neg |
−0.105 |
−0.214 |
0.005 |
0.068 |
ns |
| Distance |
Temp.Path |
35°C__pos |
28°C__neg |
−0.588 |
−0.883 |
−0.294 |
<0.001 |
**** |
| Distance |
Temp.Path |
32°C__neg |
28°C__pos |
−0.168 |
−0.267 |
−0.070 |
<0.001 |
**** |
| Distance |
Temp.Path |
32°C__pos |
28°C__pos |
−0.088 |
−0.186 |
0.010 |
0.107 |
ns |
| Distance |
Temp.Path |
35°C__neg |
28°C__pos |
−0.150 |
−0.233 |
−0.067 |
<0.001 |
**** |
| Distance |
Temp.Path |
35°C__pos |
28°C__pos |
−0.634 |
−0.919 |
−0.348 |
<0.001 |
**** |
| Distance |
Temp.Path |
32°C__pos |
32°C__neg |
0.080 |
−0.021 |
0.182 |
0.199 |
ns |
| Distance |
Temp.Path |
35°C__neg |
32°C__neg |
0.018 |
−0.068 |
0.105 |
≥0.25 |
ns |
| Distance |
Temp.Path |
35°C__pos |
32°C__neg |
−0.465 |
−0.752 |
−0.179 |
<0.001 |
*** |
| Distance |
Temp.Path |
35°C__neg |
32°C__pos |
−0.062 |
−0.149 |
0.024 |
≥0.25 |
ns |
| Distance |
Temp.Path |
35°C__pos |
32°C__pos |
−0.546 |
−0.832 |
−0.259 |
<0.001 |
**** |
| Distance |
Temp.Path |
35°C__pos |
35°C__neg |
−0.484 |
−0.765 |
−0.202 |
<0.001 |
**** |
| gunifrac |
| Distance |
Temp.Path |
28°C__pos |
28°C__neg |
0.011 |
−0.129 |
0.151 |
≥0.25 |
ns |
| Distance |
Temp.Path |
32°C__neg |
28°C__neg |
−0.091 |
−0.234 |
0.052 |
≥0.25 |
ns |
| Distance |
Temp.Path |
32°C__pos |
28°C__neg |
−0.014 |
−0.157 |
0.129 |
≥0.25 |
ns |
| Distance |
Temp.Path |
35°C__neg |
28°C__neg |
−0.129 |
−0.258 |
0.000 |
0.049 |
* |
| Distance |
Temp.Path |
35°C__pos |
28°C__neg |
−0.411 |
−0.758 |
−0.065 |
0.011 |
* |
| Distance |
Temp.Path |
32°C__neg |
28°C__pos |
−0.102 |
−0.218 |
0.014 |
0.115 |
ns |
| Distance |
Temp.Path |
32°C__pos |
28°C__pos |
−0.025 |
−0.141 |
0.091 |
≥0.25 |
ns |
| Distance |
Temp.Path |
35°C__neg |
28°C__pos |
−0.140 |
−0.238 |
−0.043 |
<0.001 |
*** |
| Distance |
Temp.Path |
35°C__pos |
28°C__pos |
−0.423 |
−0.759 |
−0.087 |
0.006 |
** |
| Distance |
Temp.Path |
32°C__pos |
32°C__neg |
0.077 |
−0.042 |
0.196 |
≥0.25 |
ns |
| Distance |
Temp.Path |
35°C__neg |
32°C__neg |
−0.038 |
−0.140 |
0.064 |
≥0.25 |
ns |
| Distance |
Temp.Path |
35°C__pos |
32°C__neg |
−0.321 |
−0.658 |
0.017 |
0.072 |
ns |
| Distance |
Temp.Path |
35°C__neg |
32°C__pos |
−0.115 |
−0.217 |
−0.013 |
0.017 |
* |
| Distance |
Temp.Path |
35°C__pos |
32°C__pos |
−0.398 |
−0.735 |
−0.060 |
0.011 |
* |
| Distance |
Temp.Path |
35°C__pos |
35°C__neg |
−0.283 |
−0.614 |
0.049 |
0.140 |
ns |
6C)

Tables
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GLM
| GLM Results |
| glm(Total.Worm.Count ~ Alpha.Score*Cluster); Exposed fish |
| term |
estimate |
std.error |
statistic |
p.value |
p.adj.sig |
| Shannon |
| (Intercept) |
0.000 |
0.778 |
0.000 |
≥0.25 |
ns |
| Alpha.Score |
0.000 |
1.808 |
0.000 |
≥0.25 |
ns |
| ClusterLow |
6.524 |
1.067 |
6.115 |
<0.001 |
**** |
| ClusterHigh |
−2.610 |
3.512 |
−0.743 |
≥0.25 |
ns |
| Alpha.Score:ClusterLow |
−10.090 |
4.999 |
−2.018 |
0.047 |
* |
| Alpha.Score:ClusterHigh |
13.706 |
4.766 |
2.876 |
0.005 |
** |
| Simpson |
| (Intercept) |
0.000 |
0.741 |
0.000 |
≥0.25 |
ns |
| Alpha.Score |
0.000 |
1.605 |
0.000 |
≥0.25 |
ns |
| ClusterLow |
6.842 |
1.041 |
6.573 |
<0.001 |
**** |
| ClusterHigh |
−3.247 |
3.900 |
−0.833 |
≥0.25 |
ns |
| Alpha.Score:ClusterLow |
−12.165 |
4.647 |
−2.618 |
0.011 |
* |
| Alpha.Score:ClusterHigh |
14.198 |
5.090 |
2.789 |
0.007 |
** |
| Richness |
| (Intercept) |
0.000 |
0.914 |
0.000 |
≥0.25 |
ns |
| Alpha.Score |
0.000 |
1.983 |
0.000 |
≥0.25 |
ns |
| ClusterLow |
6.105 |
1.294 |
4.717 |
<0.001 |
**** |
| ClusterHigh |
7.362 |
2.644 |
2.784 |
0.007 |
** |
| Alpha.Score:ClusterLow |
−3.491 |
4.463 |
−0.782 |
≥0.25 |
ns |
| Alpha.Score:ClusterHigh |
0.772 |
4.163 |
0.185 |
≥0.25 |
ns |
ANOVA
| ANOVA of GLM |
| ANOVA(GLM(Total.Worm.Count ~ Alpha.Score*Cluster), type = 2); Exposed fish |
| term |
statistic |
df |
p.value |
sig |
| Shannon |
| Alpha.Score |
0.143 |
1.000 |
≥0.25 |
ns |
| Cluster |
162.212 |
2.000 |
<0.001 |
**** |
| Alpha.Score:Cluster |
14.204 |
2.000 |
<0.001 |
*** |
| Simpson |
| Alpha.Score |
0.002 |
1.000 |
≥0.25 |
ns |
| Cluster |
168.513 |
2.000 |
<0.001 |
**** |
| Alpha.Score:Cluster |
16.419 |
2.000 |
<0.001 |
*** |
| Richness |
| Alpha.Score |
0.066 |
1.000 |
≥0.25 |
ns |
| Cluster |
145.539 |
2.000 |
<0.001 |
**** |
| Alpha.Score:Cluster |
0.741 |
2.000 |
≥0.25 |
ns |
Tukey
| Pairwise Tukey's HSD, p.adj: Dunnett |
| Tukey(Total.Worm.Count ~ Alpha.Score*Cluster); Exposed fish |
| Temperature |
.y. |
term |
group1 |
group2 |
estimate |
std.error |
statistic |
adj.p.value |
Variable |
Group |
| Shannon |
| 28 |
Alpha.Score |
Cluster |
Low |
Other |
−1.594 |
0.642 |
−2.483 |
0.034 |
Cluster |
28 |
| 28 |
Alpha.Score |
Cluster |
High |
Other |
2.844 |
0.672 |
4.233 |
<0.001 |
Cluster |
28 |
| 28 |
Alpha.Score |
Cluster |
High |
Low |
4.438 |
0.773 |
5.741 |
<0.001 |
Cluster |
28 |
| 32 |
Alpha.Score |
Cluster |
Low |
Other |
−1.391 |
0.495 |
−2.808 |
0.013 |
Cluster |
32 |
| 32 |
Alpha.Score |
Cluster |
High |
Other |
1.147 |
0.333 |
3.443 |
0.002 |
Cluster |
32 |
| 32 |
Alpha.Score |
Cluster |
High |
Low |
2.538 |
0.528 |
4.811 |
<0.001 |
Cluster |
32 |
| 35 |
Alpha.Score |
Cluster |
Low |
Other |
−0.899 |
0.659 |
−1.365 |
0.172 |
Cluster |
35 |
| Simpson |
| 28 |
Alpha.Score |
Cluster |
Low |
Other |
−1.487 |
0.656 |
−2.266 |
0.060 |
Cluster |
28 |
| 28 |
Alpha.Score |
Cluster |
High |
Other |
2.970 |
0.665 |
4.466 |
<0.001 |
Cluster |
28 |
| 28 |
Alpha.Score |
Cluster |
High |
Low |
4.457 |
0.767 |
5.810 |
<0.001 |
Cluster |
28 |
| 32 |
Alpha.Score |
Cluster |
Low |
Other |
−1.233 |
0.487 |
−2.531 |
0.029 |
Cluster |
32 |
| 32 |
Alpha.Score |
Cluster |
High |
Other |
1.264 |
0.354 |
3.573 |
<0.001 |
Cluster |
32 |
| 32 |
Alpha.Score |
Cluster |
High |
Low |
2.497 |
0.528 |
4.729 |
<0.001 |
Cluster |
32 |
| 35 |
Alpha.Score |
Cluster |
Low |
Other |
−0.779 |
0.692 |
−1.125 |
≥0.25 |
Cluster |
35 |
| Richness |
| 28 |
Alpha.Score |
Cluster |
Low |
Other |
−1.430 |
0.589 |
−2.429 |
0.040 |
Cluster |
28 |
| 28 |
Alpha.Score |
Cluster |
High |
Other |
1.367 |
0.653 |
2.095 |
0.090 |
Cluster |
28 |
| 28 |
Alpha.Score |
Cluster |
High |
Low |
2.797 |
0.692 |
4.043 |
<0.001 |
Cluster |
28 |
| 32 |
Alpha.Score |
Cluster |
Low |
Other |
−1.183 |
0.387 |
−3.052 |
0.006 |
Cluster |
32 |
| 32 |
Alpha.Score |
Cluster |
High |
Other |
0.429 |
0.278 |
1.543 |
≥0.25 |
Cluster |
32 |
| 32 |
Alpha.Score |
Cluster |
High |
Low |
1.611 |
0.410 |
3.934 |
<0.001 |
Cluster |
32 |
| 35 |
Alpha.Score |
Cluster |
Low |
Other |
−0.611 |
0.552 |
−1.107 |
≥0.25 |
Cluster |
35 |
6D)

Tables
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Capscale
Dispersion (ANOVA)
Dispersion (Tukey)
7) Gut microbial abundance is significantly associated with
environmental conditions and stressors
7A)
Fig. 7 A heatmap of model coefficient values of the top 50
statistically significant abundant gut microbial taxa identified by
MaAsLin2. The color of each cell represents the coefficient value and
direction (red is positive, blue is negative). A “+” or “-” indicates a
statistically significant association was observed between taxon
abundance and a covariate. Gray colored cells indicate a significant
effect was not observed.
| Taxon |
Temp: 32°C |
Temp: 35°C |
Time (DPE) |
Cluster: Low |
Parasite exposed |
Infection present |
Infection burden |
| Actinobacteriota |
| IMCC26207 |
+ |
|
- |
- |
- |
|
|
| Nocardiaceae Genus |
+ |
+ |
+ |
|
|
|
|
| PeM15 Genus |
+ |
|
- |
- |
- |
|
|
| Bacteroidota |
| Barnesiellaceae Genus |
|
|
- |
|
- |
|
|
| Cloacibacterium |
|
+ |
- |
|
|
|
|
| Flavobacterium |
- |
- |
- |
- |
+ |
|
|
| Fluviicola |
|
- |
- |
|
- |
- |
|
| Microscillaceae Genus |
+ |
+ |
+ |
- |
|
|
|
| Terrimonas |
|
|
|
- |
|
+ |
+ |
| env OPS 17 Genus |
|
- |
|
- |
- |
|
|
| Chloroflexi |
| JG30 KF CM45 Genus |
|
|
+ |
- |
+ |
+ |
|
| Cyanobacteria |
| Candidatus Obscuribacter |
- |
- |
+ |
- |
+ |
+ |
|
| Obscuribacteraceae Genus |
+ |
|
+ |
- |
|
+ |
- |
| Vampirovibrionaceae Genus |
|
|
+ |
- |
+ |
+ |
|
| Desulfobacterota |
| Desulfobacterota Genus |
|
|
|
- |
+ |
+ |
|
| Firmicutes |
| Clostridium sensu stricto 3 |
|
|
+ |
- |
+ |
+ |
|
| Gemmatimonadota |
| Gemmatimonadaceae Genus |
|
- |
|
- |
+ |
+ |
|
| Myxococcota |
| Sandaracinaceae Genus |
+ |
+ |
+ |
- |
+ |
+ |
|
| NB1-j |
| NB1 j Genus |
|
+ |
+ |
- |
+ |
+ |
|
| Nitrospirota |
| Nitrospira |
|
- |
+ |
- |
+ |
+ |
|
| Patescibacteria |
| Saccharimonadales Genus |
+ |
+ |
+ |
- |
+ |
|
|
| Planctomycetota |
| CL500 3 |
|
|
+ |
- |
- |
+ |
|
| Gemmata |
|
+ |
+ |
- |
+ |
+ |
|
| Pirellulaceae Genus |
+ |
- |
+ |
- |
- |
|
|
| Planctomycetales Genus |
|
|
+ |
- |
+ |
+ |
|
| Planctomycetes Genus |
|
|
+ |
- |
+ |
+ |
|
| Rhodopirellula |
|
|
|
- |
+ |
+ |
- |
| SM1A02 |
|
|
+ |
- |
+ |
+ |
|
| Telmatocola |
|
|
+ |
- |
+ |
+ |
|
| Proteobacteria |
| A0839 Genus |
+ |
|
|
- |
+ |
+ |
|
| Alphaproteobacteria Genus |
|
+ |
+ |
- |
+ |
+ |
|
| Bauldia |
+ |
|
+ |
- |
+ |
+ |
|
| Beijerinckiaceae Genus |
|
- |
+ |
- |
|
+ |
- |
| Bosea |
- |
- |
- |
- |
|
|
|
| Candidatus Alysiosphaera |
+ |
|
+ |
- |
+ |
+ |
|
| Chitinibacter |
|
+ |
+ |
|
+ |
|
- |
| Comamonadaceae Genus |
|
|
- |
- |
+ |
+ |
|
| Gammaproteobacteria Genus |
- |
- |
+ |
- |
|
+ |
|
| Luteimonas |
- |
|
|
- |
|
+ |
+ |
| Methyloligellaceae Genus |
+ |
+ |
+ |
- |
+ |
+ |
|
| Nordella |
|
- |
|
- |
+ |
+ |
|
| Plesiomonas |
+ |
+ |
|
|
- |
- |
- |
| Pseudomonadales Genus |
- |
- |
- |
- |
- |
- |
|
| Rhizobacter |
|
|
|
- |
+ |
+ |
+ |
| Rhizobiales Incertae Sedis Genus |
|
|
|
- |
|
+ |
|
| Rhodobacteraceae Genus |
|
|
|
- |
+ |
+ |
|
| Rhodovastum |
|
- |
|
- |
|
|
|
| Steroidobacteraceae Genus |
|
|
+ |
- |
+ |
+ |
|
| Xanthobacteraceae Genus |
- |
- |
+ |
- |
|
+ |
|
| Verrucomicrobiota |
| Oikopleura |
|
|
+ |
- |
+ |
+ |
|